| 鄂西南地区稻瘟病菌AVR-Pia动态变化研究 |
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| 引用本文： | 韩旭晨,董岩,韩豪杰,谭艳平,刘学群,刘新琼,徐鑫,李开,王春台.鄂西南地区稻瘟病菌AVR-Pia动态变化研究[J].中国农学通报,2022,38(21):112-121. |
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| 作者姓名： | 韩旭晨 董岩 韩豪杰 谭艳平 刘学群 刘新琼 徐鑫 李开 王春台 |
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| 作者单位： | 中南民族大学生命科学学院/生物技术国家民委重点实验室/武陵山区特色资源植物种质保护与利用湖北省重点实验室,武汉 430074 |
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| 基金项目： | 湖北省自然科学基金重点项目“武陵山区稻瘟病菌小种流行演替与抗病育种”(2015CFA015)； |
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| 摘 要： | 为了解鄂西南地区稻瘟病菌AVR-Pia的分布和动态变化,于2017—2020年在鄂西南9个地点同时种植特定100个水稻品种,采集感病稻杆并分离稻瘟病菌;利用水稻单基因系IRBLa-A进行致病性鉴定,设计无毒基因AVR-Pia特异性引物进行PCR扩增和序列分析。结果表明2017—2020年分离保存的661株稻瘟病菌菌株中,有49株含有无毒基因AVR-Pia（占7.4%）,不同年份及不同地点AVR-Pia出现频率差异明显,2017—2020年分别为7.5%、16.0%、3.1%、5.0%,AVR-Pia出现频率最高的是野三关(15.8%),其次是柏杨和来凤（都是14.6%）,咸丰、建始和太平未出现。含Pia的单基因系IRBLa-A对其中91个菌株(13.8%)具有抗性。49个菌株能扩增出463 bp的特异性条带,序列分析发现其CDS区域未发现突变,起始密码子上游109 bp位点发生碱基突变G/T,该位点变异是否与表达相关有待进一步研究。说明鄂西南地区无毒基因AVR-Pia存在较少,抗性基因Pia不适合在鄂西南地区作为主效抗病基因来选择育种。
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| 关 键 词： | 稻瘟病菌 AVR-Pia 动态变化 鄂西南地区 变异机理 |
| 收稿时间： | 2021-11-11 |
Study on Dynamic Changes of AVR-Pia of Magnaporthe grisea in Southwestern Hubei |
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| HAN Xuchen,DONG Yan,HAN Haojie,TAN Yanping,LIU Xuequn,LIU Xinqiong,XU Xin,LI Kai,WANG Chuntai.Study on Dynamic Changes of AVR-Pia of Magnaporthe grisea in Southwestern Hubei[J].Chinese Agricultural Science Bulletin,2022,38(21):112-121. |
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| Authors: | HAN Xuchen DONG Yan HAN Haojie TAN Yanping LIU Xuequn LIU Xinqiong XU Xin LI Kai WANG Chuntai |
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| Institution: | College of Life Science/Key Laboratory of State Ethnic Affairs Commission for Biological Technology/Hubei Provincial Key Laboratory for Protection and Application of Special Plants in Wuling Area of China/South-Central Minzu University, Wuhan 430074 |
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| Abstract: | In order to understand the distribution and dynamic changes of AVR-Pia of Magnaporthe grisea in southwestern Hubei, 100 specific rice varieties were simultaneously planted in 9 locations in southwestern Hubei from 2017 to 2020, and the susceptible rice straws were collected to isolate monospore. We used the rice monogenic line IRBLa-A for pathogenicity identification, and designed specific primers for the avirulent gene AVR-Pia for PCR amplifying and sequence analyzing. The results showed that among the 661 isolates of Magnaporthe grisea isolated from 2017 to 2020, 49 isolates (7.4%) contained avirulent gene AVR-Pia. The frequencies of AVR-Pia were different significantly in different years and locations. From 2017 to 2020, the frequency of AVR-Pia was 7.5%, 16.0%, 3.1% and 5.0%, respectively. The location with the highest frequency of AVR-Pia was Yesanguan (15.8%), followed by Baiyang and Laifeng (both 14.6%), and no AVR-Pia was observed in Xianfeng, Jianshi and Taiping. The monogenic line IRBLa-A with Pia was resistant to 91 of the 661 isolates (13.8%). There were 49 isolates with 463 bp specific band of AVR-Pia, and no mutation was found in the CDS region through sequence analysis, but a base mutation G/T occurred at the 109 bp upstream of the start codon. Whether this point mution was related to expression needs further study. These results indicate that the avirulent gene AVR-Pia is less in southwestern Hubei, and the resistance gene Pia is not suitable for selective breeding as a major disease resistance gene in this region. |
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| Keywords: | Magnaporthe grisea AVR-Pia dynamic changes southwestern Hubei variation mechanism |
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