| 山东核桃良种SSR指纹图谱及分子身份证的构建——基于毛细管电泳分析 |
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| 引用本文： | 梁燕,韩传明,周继磊,孙超,王翠香,李春明,王静,闵旭峰,公庆党,孟晓烨,杨绪强.山东核桃良种SSR指纹图谱及分子身份证的构建——基于毛细管电泳分析[J].中国农学通报,2022,38(15):113-121. |
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| 作者姓名： | 梁燕 韩传明 周继磊 孙超 王翠香 李春明 王静 闵旭峰 公庆党 孟晓烨 杨绪强 |
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| 作者单位： | 1.山东省林业科学研究院,济南 250014;2.山东省林业保护和发展服务中心,济南 250014;3.海阳市林业站,山东海阳 265100;4.枣庄市山亭区自然资源局,山东枣庄 277500;5.济南市长清区文昌林业站,济南 250300 |
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| 基金项目： | 山东省重点研发计划项目“核桃分子辅助杂交育种及抗病优质新品种选育”(2020LZGC0901)； |
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| 摘 要： | 通过分析山东省内核桃良种的遗传背景,并构建其分子身份证,以期为核桃品种的选育、良种的质量追溯提供技术支持。本研究以17个核桃品种为试材,利用荧光SSR分子标记方法构建其指纹图谱,在分子层面进行数据分析。17个核桃品种间Nei’s遗传距离0.181~0.720,遗传相似系数0.267~0.867;筛选出5对SSR核心引物构建品种的指纹图谱,核心引物可分成HF9+HF15,HF3+HF9+HF14两组,每组均可完全区分17个品种;以指纹图谱为基础,与商品信息结合,数字化编码生成核桃品种分子身份证。17个核桃品种遗传基础较广,其聚类结果与地理位置相关性不大;5对核心引物多态性较好,可用于构建核桃指纹图谱;编码生成的分子二维码为核桃良种鉴定提供基础。
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| 关 键 词： | 核桃 山东省 SSR 核心引物 特征指纹图谱 分子二维码 |
| 收稿时间： | 2021-08-03 |
Construction of SSR Fingerprint and Molecular Identity Card of Shandong Elite Julans regia Cultivars—Based on Capillary Electrophoresis Analysis |
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| LIANG Yan,HAN Chuanming,ZHOU Jilei,SUN Chao,WANG Cuixiang,LI Chunming,WANG Jing,MIN Xufeng,GONG Qingdang,MENG Xiaoye,YANG Xuqiang.Construction of SSR Fingerprint and Molecular Identity Card of Shandong Elite Julans regia Cultivars—Based on Capillary Electrophoresis Analysis[J].Chinese Agricultural Science Bulletin,2022,38(15):113-121. |
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| Authors: | LIANG Yan HAN Chuanming ZHOU Jilei SUN Chao WANG Cuixiang LI Chunming WANG Jing MIN Xufeng GONG Qingdang MENG Xiaoye YANG Xuqiang |
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| Institution: | 1.Shandong Academy of Forestry, Jinan 250014;2.Forestry Protection and Development Service Center of Shandong Province, Jinan 250014;3.Haiyang Forestry Station, Haiyang, Shandong 265100;4.Zaozhuang Shanting District Natural Resources Bureau, Zaozhuang, Shandong 277500;5.Wenchang Forestry Station, Changqing District, Jinan 250300 |
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| Abstract: | The study analyzed the genetic background and constructed the molecular ID cards of Shandong elite Julans regia cultivars, aiming to provide technical support for breeding elite walnut cultivars and quality traceability. In this study, 17 walnut cultivars were used as the test materials to construct the fingerprints by using fluorescent SSR markers, and the data were analyzed at the molecular level. Nei's genetic distance ranged from 0.181 to 0.720 and the genetic similarity coefficient ranged from 0.267 to 0.867 among the 17 walnut cultivars. Five pairs of SSR primers were screened to construct the fingerprints of the 17 walnut cultivars. The core primers could be divided into two groups: HF9 + HF15 and HF3 + HF9 + HF14, and each group could completely distinguish the 17 cultivars. Based on fingerprint and combined with commodity information, the molecular ID cards of the 17 walnut cultivars were established by digital coding. The genetic basis of the 17 walnut cultivars was wide, and the result showed that there was no obvious correlation between the cluster relationship and geographical location. The 5 core primers had a good polymorphism and could be used to construct walnut fingerprint. Molecular QR code could provide a basis for the identification of elite Julans regia cultivars. |
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| Keywords: | Julans regia Shandong Province SSR core primer characteristic fingerprint molecular QR code |
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