| 茶叶浸取液制备的纳米银对土壤微生物的影响 |
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| 引用本文： | 洪慈清,孙语遥,莫雯婧,方云,陈芳容,桂芳泽,关雄,潘晓鸿.茶叶浸取液制备的纳米银对土壤微生物的影响[J].中国农学通报,2022,38(23):56-63. |
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| 作者姓名： | 洪慈清 孙语遥 莫雯婧 方云 陈芳容 桂芳泽 关雄 潘晓鸿 |
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| 作者单位： | 福建农林大学闽台作物有害生物生态防控国家重点实验室&生物农药与化学生物学教育部重点实验室&海峡两岸特色作物安全生产省部共建协同创新中心,福州 350002 |
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| 基金项目： | 福建省科技厅面上项目“纳米Bt生物农药在茶叶中的空间分布与环境安全性研究”(2020J01522);福建农林大学科技发展资金“纳米材料对植物病原菌的抗菌机制研究”(CXZX2019005S),“纳米二氧化硅对加替沙星抗菌效果的影响”(CXZX2020024A);福建农林大学茶产业链科技创新与服务体系建设项目“环境友好复合生物杀虫剂”(K1520005A03) |
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| 摘 要： | 为探究茶叶浸取液制备的纳米银对土壤微生物的生物安全性的影响,本研究利用植物还原法制备纳米银抗菌材料,并将其制成1000 mg/L的纳米银喷雾,分析其对土壤微生物多样性及丰富度的影响。结果表明：空白对照组(CK)与实验组(Nano-Ag)的样本土壤中,所具有的微生物类别基本一致,但是不同类别微生物的丰度具有一定差异。实验组和对照组的Coverage数值均较高(Coverage>99.71%),香农指数均较大(Shannon>5.46),辛普森指数均较小(Simpson<0.02),两组间ACE指数和Chao指数差异均较小(ACE<2,Chao<6);同时,纳米银可抑制变形菌门下的α-变形菌纲和放线菌门下的嗜热油菌纲的生长,而能够促进绿弯菌门下纤线杆菌纲的生长。本文验证了纳米银具有一定程度的生物安全性,拓宽了纳米银材料的潜在应用范围,为纳米材料在环境及医疗等领域的深入应用奠定了基础。
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| 关 键 词： | 纳米银 生物安全性 土壤微生物 多样性 |
| 收稿时间： | 2022-04-01 |
Effects of Nano-silver Prepared from Tea Extract on Soil Microorganisms |
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| HONG Ciqing,SUN Yuyao,MO Wenjing,FANG Yun,CHEN Fangrong,GUI Fangze,GUAN Xiong,PAN Xiaohong.Effects of Nano-silver Prepared from Tea Extract on Soil Microorganisms[J].Chinese Agricultural Science Bulletin,2022,38(23):56-63. |
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| Authors: | HONG Ciqing SUN Yuyao MO Wenjing FANG Yun CHEN Fangrong GUI Fangze GUAN Xiong PAN Xiaohong |
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| Institution: | State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops & Key Laboratory of Biopesticideand Chemical Biology of Ministry of Education & Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002 |
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| Abstract: | To explore the effect of nano-silver prepared from tea extract on the biosafety of soil microorganisms, the nano-silver used as antibacterial materials were synthesized by plant reduction method, and prepared into 1000 mg/L nano-silver spray. Then, we analyzed nano-silver’s impact on soil microbial diversity and richness. The results showed that the population of microorganisms was similar in the soil samples of blank control group (CK) and experimental group (Nano-Ag), but the richness of microorganisms was different. In both the experimental group and the blank control group, the Coverage value was high (Coverage>99.71%), the Shannon index was high (Shannon>5.46), and the Simpson index was low (Simpson<0.02), while ACE index and Chao index had little difference between CK and Nano-Ag group (ACE<2, Chao<6). In addition, the nano-silver inhibited the growth of Proteobacteria (Alphaproteobacteria) and Actinobacteria (Thermoleophilia), but promoted the growth of Chloroflexi (Ktedonobacteria). This study verifies that nano-silver has a certain degree of biosafety, which will broaden the potential application scope of nano-sliver and lay a foundation for the further application of nanomaterials in environmental and medical fields. |
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| Keywords: | nano-silver biosafety soil microorganisms diversity |
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