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| 云南猪屎豆属遗传多样性的ISSR分析 | |
| 引用本文： | 刘忠辉,刘国道,黄必志,罗富成.云南猪屎豆属遗传多样性的ISSR分析[J].草业学报,2009,18(5):184-191. |
| 作者姓名： | 刘忠辉 刘国道 黄必志 罗富成 |
| 作者单位： | 1. 云南农业大学草业科学系,云南,昆明,650201 2. 中国热带农业科学院热带作物品种资源研究所 3. 云南省草地动物科学研究院,云南,小哨,650212 |
| 摘 要： | 以云南４１份野生猪屎豆属种质为材料，用ＩＳＳＲ 进行分析，结果表明，１００个ＩＳＳＲ 引物中共筛选出１６个多态性明显、反应稳定的引物，４１份材料ＤＮＡ 共扩增出１６４条条带，平均每个引物的扩增条带数为１０．３条，其中多态性条带总数为１５９条，多态性比率为９９．３％。材料间遗传相似系数范围在０．４８１７～１．００００，表现出丰富的遗传多样性；通过正交设计优化，得出适合猪屎豆属野生植物的ＩＳＳＲ－ＰＣＲ 反应体系为：４０ｎｇ 模板ＤＮＡ、引物０．２μｍｏｌ／Ｌ、２００μｍｏｌ／ＬｄＮＴＰ、１×Ｂｕｆｆｅｒ （Ｍｇ２＋ ｐｌｕｓ）、０．５ Ｕ Ｔａｑ 酶（ＴａＫａＲａ）；ＩＳＳＲ 引物最佳退火温度为５０ 或５３℃；通过聚类分析，可将４１份猪屎豆属种质分为５大类，同一种的种质可以很好的聚成一类；通过对思茅猪屎豆ＩＳＳＲ 鉴定，得出它与猪屎豆属的遗传相似系数接近，然而确定该物种属于猪屎豆属需要做更多的鉴定。 |
| 关 键 词： | 猪屎豆属 ＩＳＳＲ 遗传多样性分析 |
| 收稿时间： | 1900-01-01; |
Genetic diversity markers detected by inter-simple sequence repeats(ISSRs) in Crotalaria latifolia in Yunnan |
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| LIU Zhong-hui,LIU Guo-dao,HUANG Bi-zhi,LUO Fu-cheng.Genetic diversity markers detected by inter-simple sequence repeats(ISSRs) in Crotalaria latifolia in Yunnan[J].Acta Prataculturae Sinica,2009,18(5):184-191. | |
| Authors: | LIU Zhong-hui LIU Guo-dao HUANG Bi-zhi LUO Fu-cheng |
| Institution: | LIU Zhong-hui1,LIU Guo-dao2,HUANG Bi-zhi3,LUO Fu-cheng1 (1.Department of Grassl, Science,Yunnan Agricultural University,Kunming 650201,China,2.Tropical Crop Genetic Resources Insitute,CATAS,Danzhou 571737,3.Yunnan Academy of Grassl, , Animal Science,Xiaoshao 650212,China) |
| Abstract: | Forty-one Crotalaria latifolia wild resources in Yunnan were analyzed using inter-simple sequence repeats(ISSR).Sixteen of 100 primers were selected,and 164 DNA fragments were amplified from 41 samples to give 159 fragments(99.3%) that were polymorphic,an average of 10.3 DNA bands per primer.The genetic similarity among all accessions ranged from 0.481 7 to 1.000 0,indicating a comparatively rich genetic diversity among the tested accessions.A better PCR reaction system was obtained using an orthogonal desi... |
| Keywords: | ISSR |
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