| 污水处理反硝化除磷-诱导结晶磷回收工艺中除磷微生物特性 |
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| 引用本文： | 邹海明,吕锡武,李 婷.污水处理反硝化除磷-诱导结晶磷回收工艺中除磷微生物特性[J].农业工程学报,2014,30(5):155-161. |
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| 作者姓名： | 邹海明 吕锡武 李 婷 |
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| 作者单位： | 1. 东南大学能源与环境学院，南京 210096; 2. 安徽科技学院资源环境系，凤阳 233100;;1. 东南大学能源与环境学院，南京 210096;;1. 东南大学能源与环境学院，南京 210096; |
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| 基金项目： | 十二五国家水体污染控制与治理科技重大专项（2012ZX07101-005）；国家自然科学基金（51078074）；江苏省科技支撑计划（BS 2008667） |
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| 摘 要： | 为探究反硝化除磷-诱导结晶磷回收工艺中缺氧池污泥释磷、吸磷以及微生物特征,利用荧光原位杂交(fluorescence in situ hybridization,FISH)技术、电子扫描显微镜(scanning electron microscope,SEM)观察了微生物的数量、分布和形态;通过批次试验考察了污泥在厌氧/好氧和厌氧/缺氧2种模式下的释磷和吸磷特征。结果表明:该双污泥系统缺氧池中聚磷菌占总细菌比例的69.7%,明显高于单污泥系统中富集的聚磷菌比例,污泥中的微生物多呈杆状;厌氧/好氧、厌氧/缺氧模式下单位污泥浓度(mixed liquor suspended solids,MLSS)总吸磷量(以PO43--P计)分别为22.84、18.60 mg/g,反硝化聚磷菌(denitrifying polyphosphate-accumulating organisms,DPAO)占聚磷菌(polyphosphate-accumulating organisms,PAO)的比例为81.44%,表明在长期的厌氧/缺氧运行条件下可以富集到以硝酸盐为电子受体的反硝化聚磷菌,同时还存在着仅以氧气为电子受体的聚磷菌;通过pH值和氧化还原电位(oxidation reduction potential,ORP)的实时监测可以快速地了解污水生物处理系统中各类反应的进程,对调控工艺参数有着重要的意义。综上所述,为保证污水生物处理工艺的正常稳定运行,将微生物分析与常规的化学参数分析结合起来考察将是未来发展的必然趋势。
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| 关 键 词： | 细菌 污泥硝化 扫描电子显微技术 荧光原位杂交 聚磷菌 反硝化聚磷菌 |
| 收稿时间： | 8/4/2013 12:00:00 AM |
| 修稿时间： | 2013/12/27 0:00:00 |
Characteristics of phosphorus removal microorganisms in denitrifying phosphorus removal and induced crystallization phosphorus recovery process for waste water treatment |
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| Institution: | 1. School of Energy and Environment, Southeast University, Nanjing 210096, China; 2. Department of Resource and Environment, Anhui Science and Technology University, Fengyang 233100, China;;1. School of Energy and Environment, Southeast University, Nanjing 210096, China;;1. School of Energy and Environment, Southeast University, Nanjing 210096, China; |
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| Abstract: | Abstract: A novel wastewater treatment process in our previous research can perform denitrifying simultaneous nitrogen and phosphorus removal and phosphorus recovery via induced crystallization (thereafter called BNR-IC process). Generally, removal efficiencies of contaminants including carbon, nitrogen and phosphorus through wastewater biological treatment process may be attributed to characteristics of predominant microorganisms in activated sludge. In this BNR-IC process, microorganism enriched in the anoxic tank is one of key factors influencing the removal of carbon, nitrogen and phosphorus from wastewater. The aim of this study was to investigate the characteristics of activated sludge from the anoxic tank in the BNR-IC process, including the performances of anaerobic phosphorus release, aerobic or anoxic phosphorus uptake, and the characteristics of microorganisms responsible for denitrifying simultaneous nitrogen and phosphorus removal. In this study, two batch tests were conducted to analyze the capacity of release phosphorus in anaerobic condition and uptake phosphorus under aerobic or anoxic conditions. Fluorescence in situ hybridization (FISH), scanning electron micrograph (SEM) and chemical staining were also utilized to investigate the amount of polyphosphate-accumulating organisms (PAO) in activated sludge and its morphologies. FISH results showed that the ratio of polyphosphate-accumulating organisms (PAO) to total bacteria was 69.7% higher than that of single-sludge system. A variety of bacilli were found in the activated sludge samples by SEM. The amount of phosphorus taken up per MLSS, i.e. PO43--P/MLSS, in anaerobic-aerobic environment was 22.84 mg/g. It was 18.60 mg/g in anaerobic-anoxic condition, the ratio of DPAO to PAO in anoxic tank was 81.44%. The results also indicated that DPAO can be effectively enriched in a long-term operation under anaerobic-anoxic environment. PAO can be divided into two types: one uses nitrate or oxygen as an electron acceptor, and the other can only use oxygen as the electron acceptor. Moreover, better understanding of the biochemical processes in wastewater biological treatment process can be obtained by monitoring the value of pH or ORP, providing the benefit for controlling the process. These results showed that the combination of chemical analysis with microorganism investigation may serves as a new indicator for stable operation or application of the novel BNR-IC process. It may become a new trend in the future development of wastewater biological treatment and phosphorus recovery process. |
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| Keywords: | bacteria sludge digestion scanning electron microscopy fluorescence in situ hybridization (FISH) polyphosphate-accumulating organisms (PAO) denitrifying polyphosphate-accumulating organisms (DPAO) |
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