| 牛结核γ-干扰素ELISA检测方法在检疫工作中的应用 |
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| 引用本文： | 周培校,李 静,张鲁安,李 杰,曹 瑞,张喜悦,李 岩.牛结核γ-干扰素ELISA检测方法在检疫工作中的应用[J].中国动物检疫,2014,31(1):67-71. |
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| 作者姓名： | 周培校 李 静 张鲁安 李 杰 曹 瑞 张喜悦 李 岩 |
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| 作者单位： | [1]新疆兵团第八师兽医站,新疆石河子832000 [2]新疆兵团畜牧兽医总站,新疆乌鲁木齐830063 [3]青岛瑞尔生物技术有限公司,山东青岛266100 [4]中国动物卫生与流行病学中心,山东青岛266109 |
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| 摘 要： | 为评价牛γ-干扰素ELISA检测方法检测牛结核的效果及国产试剂盒的检测效果，本试验首先将国产试剂盒与Prionics试剂盒对42份相对阳性的样品和105份相对阴性的样品进行对比研究。然后对5个规模化牛场的3000头奶牛首先进行国产单纯结核菌素颈部皮内变态反应试验，3天后选取皮内变态反应阳性和可疑及部分阴性牛共418头，进行牛γ-干扰素试验。结果国产试剂盒对阳性和阴性样品的检测敏感性和特异性分别为95.2%和100%，与Priobics试剂盒的符合率为99.3%。表明国产试剂盒与进口试剂盒的检测能力一致，牛γ-干扰素检测方法准确可靠。5个牛场的3000头奶牛单纯结核菌素颈部皮内变态反应试验阳性为138头，可疑105头。γ-干扰素试验对418头奶牛的检测，其中阳性74头，与颈部皮内变态反应（可疑牛暂时视为阴性）的符合率为60.5%。
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| 关 键 词： | 牛结核 γ- 干扰素 ELISA 检疫 |
The Application of Gamma Interferon ELISA in Bovine Tuberculosis Detection |
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| Zhou Peixiao,Li Jing,Zhang Luan,Li Jie,Cao Rui,Zhang Xiyue,Li Yan.The Application of Gamma Interferon ELISA in Bovine Tuberculosis Detection[J].China Journal Of Animal Quarantine,2014,31(1):67-71. |
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| Authors: | Zhou Peixiao Li Jing Zhang Luan Li Jie Cao Rui Zhang Xiyue Li Yan |
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| Institution: | 2 ( 1.The 8 Division Vet station of Xinjiang Production and Construction Corps, Shi Hezi 832000; 2.Xinjiang Production and ConstructionCorps Animal Husbandry and Veterinary Station, Ummqi 830063 ; 3.Qingdao Real BIO- Technology Co., Ltd, Qing Dao 266100; 4. China Animal Health and Epidemiology Center, Qing Dao 266109) |
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| Abstract: | In order to evaluate the effectiveness of gamma interferon test to detect bovine tuberculosis and the detection effect of a domestic kit, a comparative study was done between Prionics kits and the domestic kits using 42 positive samples and 105 negative samples. Then 3000 cattle from five intensive farms were first tested with single skin test, and 3 days later, 418 cattle including skin test positive and suspicious ones and some negative ones were tested with gamma interferon test. Results showed that the sensitivity and specificity of the domestic kit were 95.2%and 100%respectively. and the coincidence was 99.3%with the Prionics kit,suggesting that the gamma interferon test was accurate and reli-able for detection of bovine tuberculosis, and the domestic kit was as good as the Prionics kit. There were 138 skin test positive cattle and 105 suspicious cattle from 3000 tested cattle. In gamma interferon test, 74/418 cattle were detected positive, and the coincidence rate was 60.5%with skin test （suspicious cattle considered as negative）. |
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| Keywords: | bovine tuberculosis γ- interferon ELISA： quarantine inspection |
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