| 副溶血弧菌tlh基因缺失株的构建 |
| |
| 引用本文： | 赵永刚,唐小千,战文斌.副溶血弧菌tlh基因缺失株的构建[J].上海交通大学学报(农业科学版),2011,29(1):10-15. |
| |
| 作者姓名： | 赵永刚 唐小千 战文斌 |
| |
| 作者单位： | 1. 中国海洋大学,教育部海水养殖重点实验室,青岛,266003;中国动物卫生与流行病学中心,青岛,266032
2. 中国海洋大学,教育部海水养殖重点实验室,青岛,266003 |
| |
| 摘 要： | 副溶血弧菌(Ⅵbrio parahaemolyticuss,VP)是我国海水养殖鱼、虾、贝类的重要病原,其主要致病因子热不稳定性溶血素(thermolabile hemolysin,TLH)的功能和致病性仍不十分清楚.本研究利用PCR技术从VP临床分离株的基因组DNA中扩增出tlh基因,利用同源重组技术,构建了副溶血弧...
|
| 关 键 词： | 副溶血弧菌 tlh基因 同源重组 基因敲除 溶血活性 |
Construction of tlh Gene Deleted Mutant of Vibrio parahaemolyticus |
| |
| ZHAO Yong-gang,TANG Xiao-qian,ZHAN Wen-bin.Construction of tlh Gene Deleted Mutant of Vibrio parahaemolyticus[J].Journal of Shanghai Jiaotong University (Agricultural Science),2011,29(1):10-15. |
| |
| Authors: | ZHAO Yong-gang TANG Xiao-qian ZHAN Wen-bin |
| |
| Institution: | ZHAO Yong-gang1,2,TANG Xiao-qian1,ZHAN Wen-bin1(1.Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China,2.China Animal Health and Epidemiology Center,Qingdao 266032,China) |
| |
| Abstract: | Vibrio parahaemolyticus(VP) is listed as one of the pathogenic vibrios endangering net-cage cultured Pseudosciaena crocea,Fennerpenaeus chinensis,and shellfish in coastal areas of China.TLH(thermolabile hemolysin) is one of major virulence factors produced by VP.The information regarding the biological function and nosogenesis remains completely lacking.In this study,we cloned tlh gene from the genome DNA of VP by polymerase chain reaction(PCR).Targeting vector of tlh gene was constructed by homologous reco... |
| |
| Keywords: | Vibrio parahaemolyticus TLH homologous recombination gene knockout hemolysis activity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《上海交通大学学报(农业科学版)》浏览原始摘要信息 |
| 点击此处可从《上海交通大学学报(农业科学版)》下载免费的PDF全文 |
|
