| 鸭茅叶绿体引物筛选 |
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| 引用本文： | 安明珠,赵世伟,朱文露,韩博,文亦芾,周凯.鸭茅叶绿体引物筛选[J].草学,2022(1):30-36,47. |
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| 作者姓名： | 安明珠 赵世伟 朱文露 韩博 文亦芾 周凯 |
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| 作者单位： | 云南农业大学动物科技学院,云南 昆明 650201 |
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| 基金项目： | 云南省基础研究青年项目(2019FD098)资助。 |
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| 摘 要： | 为筛选适用于鸭茅物种的基因引物序列,开展相关系统发育学研究,通过搜集鸭茅近缘物种叶绿体基因引物,使用PCR扩增技术和琼脂糖凝胶电泳检测来筛选出目的基因片段,对目的基因进行BLAST序列比对.结果表明:试验搜集的35对引物中共能筛选出trnL-F 1、trnL-F 5、rpL162、rpL164、matK 6、matK ...
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| 关 键 词： | 鸭茅 PCR 引物筛选 测序 |
Screening of Dactylis Chloroplast Primers |
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| An Mingzhu,Zhao Shiwei,Zhu Wenlu,Han Bo,Wen Yifu,Zhou Kai.Screening of Dactylis Chloroplast Primers[J].Prataculture & Animal Husbandry,2022(1):30-36,47. |
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| Authors: | An Mingzhu Zhao Shiwei Zhu Wenlu Han Bo Wen Yifu Zhou Kai |
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| Institution: | (College of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China) |
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| Abstract: | The aim of this study was to screen the primers for the phylogenetic study of Dactylis glomerata L.species.By collecting the chloroplast gene primers of the related species of Dactylis,PCR amplification technology and agarose gel electrophoresis detection were used to screen out the target gene fragments,and BLAST sequence alignment of the target gene was performed.The results showed that a total of 7 pairs of primers were screened out from the 35 pairs of primers collected in the experiment could screen out pairs of primers trnL-F 1,trnL-F 5,rpL162,rpL164,matK 6,matK 7 and rbcL 1,and the amplified bands were unique and clear.The amplified products were verified as target genes after sequencing and comparison.Seven primers of chloroplast genes screened in this study have good amplification effect and are suitable for phylogenetic analysis of Dactylis glomerata. |
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| Keywords: | Dactylis glomerata L PCR primer screening sequencing |
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