| 小麦叶片谷氨酰胺合成酶的分离纯化及鉴定 |
| |
| 引用本文： | 王小纯,安 帅,熊淑萍,程振云,陈新建,孟凡荣,马新明.小麦叶片谷氨酰胺合成酶的分离纯化及鉴定[J].麦类作物学报,2010,30(1):83-86. |
| |
| 作者姓名： | 王小纯 安 帅 熊淑萍 程振云 陈新建 孟凡荣 马新明 |
| |
| 作者单位： | 1. 河南农业大学生命科学院,河南郑州,450002
2. 河南农业大学农学院,河南郑州,450002 |
| |
| 基金项目： | 国家自然基金项目（30771266）；农业部公益项目（200705）。 |
| |
| 摘 要： | 为了解谷氨酰胺合成酶（Glutamine synthetase，GS）同工酶在小麦中的种类、活性及亚基组成状况，利用盐析、凝胶过滤、阴离子交换层析等技术对小麦苗期叶片中的GS同工酶进行了分离纯化及鉴定。通过测定GS活性跟踪GS的纯化过程，利用NativePAGE结合活性染色技术检测GS的纯化效果，利用SDSPAGE鉴定GS的亚基组成。结果表明，在小麦幼苗叶片中检测到3种GS同工酶GS1、GS2和GSx，其中Gsx含量少，但活性高。从小麦幼苗叶片中纯化得到2个GS同工酶即GS1和GS2。GS1由39 kD的亚基组成，GS2由45 kD的亚基组成，没有纯化得到GSx。
|
| 关 键 词： | 小麦 幼苗叶片 谷氨酰胺合成酶 纯化和鉴定 |
Purification and Identification of Glutamine Synthetase in Wheat Seedlings |
| |
| WANG Xiao chun,AN Shuai,XIONG Shu ping,CHENG Zhen yun,CHEN Xin jian,MENG Fan rong,MA Xin ming.Purification and Identification of Glutamine Synthetase in Wheat Seedlings[J].Journal of Triticeae Crops,2010,30(1):83-86. |
| |
| Authors: | WANG Xiao chun AN Shuai XIONG Shu ping CHENG Zhen yun CHEN Xin jian MENG Fan rong MA Xin ming |
| |
| Institution: | 1. College of Life Science, Henan Agricultural University, Zhengzhou, Henan 45002, China;
2. College of Agronomy, Henan Agricultural University, Zhengzhou, Henan 45002, China |
| |
| Abstract: | To confirm the role of glutamine synthetase isoforms in the nitrogen assimilation of wheat,three glutamine synthetase isoforms,GS1,GS2 and GSx in the wheat leaf during seedling period were purified with salting-out,gel filtration chromatography and anion-exchange chromatography.The purification process was traced by determining the GS activity of each fraction,and the purification effect was examined by Native-PAGE combined with activity staining.The subunit component of GS isoforms was analyzed by SDS-PAGE... |
| |
| Keywords: | Wheat Seedling leaf Glutamine synthetase Gurification and identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《麦类作物学报》浏览原始摘要信息 |
| 点击此处可从《麦类作物学报》下载免费的PDF全文 |
|
