| 拟南芥突变体在磷饥饿反应研究中的应用 |
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| 引用本文： | 常胜合,舒海燕,秦广雍,吴玉萍,赵海珍.拟南芥突变体在磷饥饿反应研究中的应用[J].中国农学通报,2005,21(8):196-196. |
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| 作者姓名： | 常胜合 舒海燕 秦广雍 吴玉萍 赵海珍 |
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| 作者单位： | 1. 郑州大学离子束生物工程实验室,郑州,450056
2. 郑州大学生物工程系,郑州,450001 |
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| 基金项目： | 国家“十五”科技攻关项目“离子束应用技术研究”（2001BA302B）. |
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| 摘 要： | 磷是构成生命的重要元素之一，也是土壤中有效性最低的一种营养元素。农作物的产量常受到缺磷的影响而受损。研究植物对磷饥饿的反应对于培育耐低磷农作物、减轻农民大量施磷肥的负担具有重要意义。将单基因拟南芥突变体与野生型进行比较是从分子到生理研究植物功能的一种理想方法。目前广泛应用的突变体主要有磷转运功能缺陷突变体、有机酸分泌功能缺失突变体、为研究某一特定基因功能而创造的突变体和根部形态突变体四类。它们在研究植物体内磷的转运、代谢、对磷的吸收和验证基因功能等方面发挥了重要作用。但是，在植物对磷饥饿的反应中，磷的跨液泡膜运输、植物对磷的具体调控机制等重要问题目前还没有明确的结果。如何创造针对性强的筛选方法，诱导筛选更多的专一突变体是将来解决这些问题的一个基本途径。
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| 关 键 词： | 磷饥饿 突变体 拟南芥 反应 |
| 收稿时间： | 2005-04-04 |
| 修稿时间： | 2005-04-042005-04-05 |
Arabidopsis Mutants Used in Researches on Plant Responses to Phosphate Starvation |
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| Chang Shenghe,Shu Haiyan,Qin Guangyong,Wu Yuping,Zhao Haizhen.Arabidopsis Mutants Used in Researches on Plant Responses to Phosphate Starvation[J].Chinese Agricultural Science Bulletin,2005,21(8):196-196. |
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| Authors: | Chang Shenghe Shu Haiyan Qin Guangyong Wu Yuping Zhao Haizhen |
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| Institution: | (1The Laboratory of Ionbeam Biotechnology, Physic College, Zhengzhou University, Zhengzhou 450056; 2Bioengineering Department, Zhengzhou University, Zhengzhou 450001) |
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| Abstract: | Phosphate is one of the most important elements needed by all of the lives. Meanwhile, it is also one of the least available nutrients in the soil. The yields of the crops are always affected by phosphate starvation. Research on the phosphate-starvation responses of the plants will play a key role in cultivating phosphate-resistant crops and reducing the phosphate added to soils. Comparisons of single gene mutants with the wild-type seedlings can provide an understanding of plant functions from molecular to the physiological. Phosphate-transport-function deficient mutants, organic-acid-production deficient mutants, mutants screened for identification the functions of a certain gene and mutants with altered roots were the four main types used. They have played important roles in research phosphate utilizing from soil, phosphate transportation, phosphate metabolism and identification the functions of genes. However, some fields such as the concrete regulation mechanism of plant's reaction to Pi deficiency, the Pi transportation across tonoplast are not clear until now. Creating specific screening methods, screening more responses-specific mutants will provide breakthroughs for solving out these problems. |
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| Keywords: | Phosphate starvation Mutants Arabidopsis Responses |
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