| 坏死梭杆菌斑马鱼模型建立与LD50的测定 |
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| 引用本文： | 曹阅,陈立志,刘晓颖,冯二凯,汪孙杰,徐晶,冯卓.坏死梭杆菌斑马鱼模型建立与LD50的测定[J].特产研究,2012,34(1):14-18. |
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| 作者姓名： | 曹阅 陈立志 刘晓颖 冯二凯 汪孙杰 徐晶 冯卓 |
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| 作者单位： | 1. 中国农业科学院特产研究所吉林省特种经济动物分子生物学省部共建实验室,吉林吉林,132109
2. 中国农业科学院特产研究所吉林省特种经济动物分子生物学省部共建实验室,吉林吉林132109;江苏科技大学生物与环境工程学院,江苏镇江212018 |
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| 基金项目： | 吉林省科技发展计划项目(20110223);吉林省科技发展计划项目(201205028) |
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| 摘 要： | 建立斑马鱼人工感染坏死梭杆菌动物模型。牛源坏死梭杆菌FN(AB)株以3×1010cfu/mL、3×109cfu/mL、3×108cfu/mL、3×107cfu/mL不同菌量,腹腔接种斑马鱼,定时观察斑马鱼的发病和死亡情况,建立动物感染模型。FN(AB)对斑马鱼的半数致死量(LD50)为1.06×106cfu/尾;感染发病鱼呈现出侧游、腹水、注射部位出血红肿等症状,肝脏、肾脏、肠道出现明显的病理学变化。斑马鱼可作为坏死梭杆菌致病性研究的动物模型。
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| 关 键 词： | 坏死梭杆菌 斑马鱼 动物模型 半数致死量 |
Establishment of Zebrafish Model with Fusobacterium Necrophorum Biotype AB and Determination of LD50 |
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| CAO Yue
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CHEN Li-zhi
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LIU Xiao-ying
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FENG Er-kai
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WANG Sun-jie
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XU Jing
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FENG Zhuo.Establishment of Zebrafish Model with Fusobacterium Necrophorum Biotype AB and Determination of LD50[J].Special Wild Economic Animal and Plant Research,2012,34(1):14-18. |
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| Authors: | CAO Yue CHEN Li-zhi LIU Xiao-ying FENG Er-kai WANG Sun-jie XU Jing FENG Zhuo |
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| Institution: | 1(1.Institute of Special Wild Economic Animals and Plants,CAAS,State Key Laboratory of Special Economic Animal Molecular Biology,Jilin 132109,china;2.College of Biological and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang 212018,China) |
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| Abstract: | To establish zebrafish model infected with Fusobacterium Necrophorum Biotype FN(AB) artificially,zebrafish were divided,and were given intraperitoneal injection with FN(AB) isolate from cattle in different dosages of 3×1010cfu/mL,3×109cfu/mL,3×108cfu/mL,3×107cfu/mL,respectively.The morbidity and mortality of zebrafish were monitored in fixed time to establish the infectious animal model.The 50% lethal dose of FN(AB) in zebrafish(LD50) was 1.06 × 106 cfu / mL.Side swimming,ascites,red and swollen in the injection site was observed in the infected Zebrafish.There were also obvious pathological changes in liver,renal,and intestinal tract of the infected zebrafish.Therefore,zebrafish can be an animal model infected with FN(AB). |
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| Keywords: | Fusobacterium necrophorum Zebrafish Animal model 50% lethal dose |
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