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中国西南区扁穗牛鞭草种质遗传多样性的SRAP分析
引用本文:范彦,徐远东,蒋安,王平.中国西南区扁穗牛鞭草种质遗传多样性的SRAP分析[J].广西农业生物科学,2010(1):63-70.
作者姓名:范彦  徐远东  蒋安  王平
作者单位:重庆市畜牧科学院;
基金项目:重庆市重大专项项目(CSTC,2009AA1008);;国家科技支撑计划项目(2008BADB3B10-2)共同资助
摘    要:本研究采用SRAP标记对主要来自中国西南地区(四川,重庆,贵州和云南)的43份扁穗牛鞭草种质资源的遗传多样性进行了分析。试验筛选出了11对引物组合对43份供试材料进行扩增,共获得153条带,其中多态性条带140条,多态性条带比率为91.50%,平均每对引物扩增出条带13.91,多态性条带12.73。实验数据结果表明,43份扁穗牛鞭草材料间的遗传相似系数(GS)为0.565~0.992,平均值为0.723,表现出了丰富的遗传多样性。聚类分析结果表明,各供试材料间的聚类与其地理来源以及形态特征类型具有一定的相关性。同时,主成分分析结果能够直观的反映了各种质间的遗传关系。5个扁穗牛鞭草地理类群间的分子方差分析(AMOVA)揭示了供试的扁穗牛鞭草总遗传变异的85.99%存在于类群内,仅有14.01%的变异存在于类群之间,类群间的分化系数ΦST=0.140。本研究结果为扁穗牛鞭草种质的收集、利用及育种提供了理论依据。

关 键 词:扁穗牛鞭草  中国西南区  遗传多样性  SRAP

Genetic Diversity of Hemarthria compressa Germplasms in Southwest China by SRAP Analysis
Fan Yan Xu Yu,ong Jiang An Wang Ping ChongQing Academy of Animal Sciences,Chongqing.Genetic Diversity of Hemarthria compressa Germplasms in Southwest China by SRAP Analysis[J].Journal of Guangxi Agricultural and Biological Science,2010(1):63-70.
Authors:Fan Yan Xu Yu  ong Jiang An Wang Ping ChongQing Academy of Animal Sciences  Chongqing
Institution:Fan Yan Xu Yu,ong Jiang An Wang Ping ChongQing Academy of Animal Sciences,Chongqing,400015
Abstract:In this paper,we have investigated and analyzed the genetic diversity of 43 Hemarthria compressa accessions mainly from Southwest China(Sichuan,Chongqing,Guizhou and Yunnan province)by using SRAP(sequence-related amplified polymorphism) technology. We have selected 11 primer pairs to identify the polymorphism among the 43 tested materials. and acquired 153 DNA fragments,140 distinguishable bands of which were amplified that accounted for 91.5% polymorphism. The average number of bands of each tested primer ...
Keywords:Hemarthria compressa  Southwest China  Genetic diversity  SRAP  
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