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| 大口鲇与鲇及其杂种F1的蛋白质和同工酶电泳图谱的比较 | |
| 引用本文： | 郑蓓蓓,邹桂伟,王朝明,罗相忠,方耀林,许映芳.大口鲇与鲇及其杂种F1的蛋白质和同工酶电泳图谱的比较[J].华中农业大学学报,2006,25(3):281-285. |
| 作者姓名： | 郑蓓蓓 邹桂伟 王朝明 罗相忠 方耀林 许映芳 |
| 作者单位： | 1. 华中农业大学水产学院,武汉,430070;农业部淡水鱼类种质资源与生物技术重点开放实验室,中国水产科学研究院长江水产研究所,荆州,434000;中国水产科学研究院淡水渔业研究中心,无锡,214081 2. 农业部淡水鱼类种质资源与生物技术重点开放实验室,中国水产科学研究院长江水产研究所,荆州,434000;中国水产科学研究院淡水渔业研究中心,无锡,214081 3. 农业部淡水鱼类种质资源与生物技术重点开放实验室,中国水产科学研究院长江水产研究所,荆州,434000 |
| 基金项目： | 农业部农业结构调整重大技术研究专项基金 |
| 摘 要： | 采用聚丙烯酰胺水平平板电泳法研究了大口鲇、鲇及其杂种F1的血清蛋白、肌肉蛋白以及肝、肾、脾、心、眼和肌肉等6种组织的醇脱氢酶(ADH)、乳酸脱氢酶(LDH)和酯酶(EST),分析了杂种F1及其与父母本的亲缘关系。结果表明,在LDH和ADH上,杂种F1与母本大口鲇相近。对比发现,肾脏和脾脏的EST同工酶电泳图谱可作为鉴别别大口鲇的生化遗传标记。 |
| 关 键 词： | 大口鲇 鲇 杂种F1 蛋白质 同工酶 |
| 文章编号： | 1000-2421（2006）03-0281-05 |
| 收稿时间： | 10 8 2005 12:00AM |
| 修稿时间： | 2005-10-082006-04-10 |
Comparative Study on the Electrophorgram of Isozymes and Proteins of Silurus meridionalis, S. asotus and Their Hybrid |
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| ZHENG Bei-bei,ZOU Gui-wei,WANG Chao-ming,LUO Xiang-zhong,FANG Yao-lin,XU Ying-fang.Comparative Study on the Electrophorgram of Isozymes and Proteins of Silurus meridionalis, S. asotus and Their Hybrid[J].Journal of Huazhong Agricultural University,2006,25(3):281-285. | |
| Authors: | ZHENG Bei-bei ZOU Gui-wei WANG Chao-ming LUO Xiang-zhong FANG Yao-lin XU Ying-fang |
| Institution: | 1,Fisheries College, Huazhong Agricultural University ,Wuhan 430070, China; 2, Key Lab of Freshwater Fish Germplasm Resources and Biotechnology of Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Jingzhou 434000, China ; 3, Fresh water Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China |
| Abstract: | This study used plane polyacrylamide gel electrophoresis,serum and muscle proteins and the polymorphism of three isozymes including alcoholdehydrogenase(ADH),lactate dehydrogenase(LDH) and esterase(EST) from six tissues,liver,kidney,spleen,heart,eye and muscle,of adult Silurus meridionalis,S.asotusand their hybrids were profiled and determined respectively.The relationship of S.meridionalis,S.asotusand their hybrid were studied.Comparison showedthat the hybrid was closer to the maternal parent,S.meridionalisthan to the other on LDH and ADH.They were monopolymorphic in the electrophorgramofprotein.In contrast,the specificities of species and tissues were detectedandthe polymorphism was observedin the electrophorgramof isozymes.Those species-specific EST bands detected in kidney and spleencould serve as the biochemical genetic markers of distinguishing S.meridionalis,S.asotusand their hybrid. |
| Keywords: | Silurusmeridionalis Silurusasotus the hybrid proteins isozymes |
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