| 氟对体外培养成骨细胞骨钙素基因表达的调控 |
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| 引用本文： | 王梅,于福清,吴培福,赵德明,苏敬良,韩博.氟对体外培养成骨细胞骨钙素基因表达的调控[J].中国农业科学,2009,42(7):2629-2632. |
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| 作者姓名： | 王梅 于福清 吴培福 赵德明 苏敬良 韩博 |
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| 作者单位： | 1. 中国农业大学动物医学院,北京,100193
2. 全国畜牧兽医总站,北京,100026 |
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| 基金项目： | 国家自然科学资金资助项目(NSFC) |
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| 摘 要： | 【目的】探讨氟对成骨细胞骨钙素(OCN)基因表达的影响。【方法】应用酶消化法分离初生小鼠头盖骨成骨细胞，取第3代成骨细胞，加入不同浓度（0～10-3mol?L-1）氟化钠，提取细胞总RNA，通过实时荧光定量PCR方法，观察成骨细胞OCN基因表达的变化。【结果】各浓度NaF均能刺激体外培养成骨细胞OCN基因的表达呈剂量依赖关系，当浓度为5×10-4mol?L-1时OCN基因表达量的增加最为明显，约为对照组的30倍。【结论】各浓度的NaF均能在基因水平刺激OCN的表达，OCN在氟中毒所致的骨相损害中起着重要的作用。
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| 关 键 词： | 氟化钠" target="_blank">face="Verdana">氟化钠 成骨细胞 骨钙素 荧光定量PCR |
| 收稿时间： | 2008-12-08; |
Regulation of Fluoride on Osteocalcin Gene Expression in Osteoblasts Cultured in vitro |
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| WANG Mei,YU Fu-qing,WU Pei-fu,ZHAO De-ming,SU Jing-liang,HAN Bo.Regulation of Fluoride on Osteocalcin Gene Expression in Osteoblasts Cultured in vitro[J].Scientia Agricultura Sinica,2009,42(7):2629-2632. |
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| Authors: | WANG Mei YU Fu-qing WU Pei-fu ZHAO De-ming SU Jing-liang HAN Bo |
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| Institution: | (College of Veterinary Medicine, China Agricultural University) |
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| Abstract: | 【Objective】 The objective of the study was to evaluate the role of fluoride on osteocalcin (OCN) gene expression in osteoblasts cultured in vitro.【Method】Osteoblasts were isolated from calvaria in neonatal mice and exposure to different doses of sodium fluoride (0-10-3mol?L-1). RNA was isolated from osteoblasts cultured in vitro and osteocalcin gene expression at different fluoride concentrations were detected by real-time fluorescent quantitative PCR. 【Result】 The results indicated that treatment of the cells with sodium fluoride (10-7-10-3mol?L-1) up-regulated the expression of osteocalcin gene in a dose-dependent manner. Furthermore, 5×10-4 mol?L-1 sodium fluoride treatment showed a maximal stimulation, which was almost 30 times higher than the control. 【Conclusion】 The results in the current study suggest that sodium fluoride stimulated OCN gene expression, and OCN may play a significant role in skeletal fluorosis. |
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| Keywords: | sodium fluoride osteoblast OCN fluorescent quantitative PCR |
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