| 丁(鱼岁)养殖群体遗传多样性的ISSR分析 |
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| 引用本文： | 关建义,杨浩,陈欣,陈宏喜,杨太有.丁(鱼岁)养殖群体遗传多样性的ISSR分析[J].安徽农业科学,2009,37(18):8367-8369. |
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| 作者姓名： | 关建义 杨浩 陈欣 陈宏喜 杨太有 |
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| 作者单位： | 河南师范大学生命科学学院,河南新乡,453007;新乡医学院生命科学技术系,河南新乡,453003;河南师范大学生命科学学院,河南新乡,453007 |
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| 基金项目： | 河南省科技攻关项目，河南省教育厅自然科学研究项目，河南省动物学重点学科项目 |
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| 摘 要： | 目的]分析丁鱼]岁]养殖群体的遗传多样性水平，为其种质资源的评价、保护和合理利用提供依据。方法]利用简单重复序列区间扩增多态性（ISSR）分子标记技术。结果]选用77个随机引物对其20个个体的基因组DNA进行PCR扩增，有18个引物可以扩增出稳定且清晰的条带，共扩增出115条稳定的DNA位点，片段大小在100-2000bp，其中多态性位点14个，多态位点比例为12.17％。个体间遗传距离为0-0.1376，平均为0.0329±0.0056。结论]丁鱼]岁]群体的遗传多样性水平较低。
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| 关 键 词： | 丁(鱼岁) 遗传多样性 ISSR |
ISSR Analysis of Genetic Diversity of Tinca tinca Cultured Population |
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| Institution: | GUAN Jian-yi et al (College of Life Sciences, Henan Norrnai University, Xinxiang, Henan 453007) |
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| Abstract: | Objective] The study analyzed the genetic diversities level of Tinca tinca cultured population,which provided reference base for the evaluation,protection and utilization of its germplasm resources.Method] The experiment was carried out by Inter-Simple Sequence Repeat(ISSR) of molecular marker technique.Result] 20 genome DNA were selected to PCR amplify from 77 ones,18 ISSR primers can be amplified the stable stripes including 115 stable DNA sites and the fragments ranging from 100 to 2 000 bp,which had 14 polymorphism loci and the percentage of polymorphic loci was 12.17%.The genetic distance between individuals was from 0 to 0.137 6,and the average genetic distance was 0.032 9±0.005 6.Conclusion] The results showed that the genetic diversity level of Tinca tinca was low. |
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| Keywords: | ISSR |
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