Inter-MITE polymorphisms of a newly identified MITE show relationships among sugarcane (Saccharum) species |
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| Authors: | Shigeki Nakayama |
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| Institution: | 1. National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602, Japan
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| Abstract: | Sugarcane, one of the most important tropical crops, belongs the genus Saccharum. This genus consists of six species, four cultivated and two wild. The domestication histories of the four cultivated Saccharum species is an interesting and important topic of study. Previous studies have categorized the four cultivated species into two groups, one consisting only of S. edule and the other comprising S. officinarum, S. sinense and S. barberi. All four species have inherited the genomic DNA of S. robustum, one of two wild relative species. Saccharum species have large genomes with complex structures, as evidenced by chromosomes with a high degree of polyploidy, alloploidy and aneuploidy. Miniature inverted-repeat transposable elements (MITEs) are class II (DNA) transposons that disperse throughout the plant genome. In this study, a Tourist family MITE sequence with 18/19-bp terminal inverted repeats and a 2-bp target site duplication was newly identified from genomic DNA of S. robustum. The abundant accumulation of this MITE sequence in the sugarcane genome enabled the application of inter-MITE polymorphism (IMP) analysis to Saccharum. IMP analysis revealed the genetic relationships among all six Saccharum species and the domestication histories of the four cultivated species. |
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