| 武昌罗索线虫线粒体基因组分析 |
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| 引用本文： | 何芳,姜爱兰,赵娜娜,刘卉,王国秀.武昌罗索线虫线粒体基因组分析[J].中国生物防治,2010(1):66-72. |
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| 作者姓名： | 何芳 姜爱兰 赵娜娜 刘卉 王国秀 |
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| 作者单位： | 华中师范大学生命科学学院,武汉430079; |
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| 基金项目： | 国家自然科学基金(30870350,30470246) |
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| 摘 要： | 采用滚环复制和两步长PCR扩增相结合的方法,成功测出武昌罗索线虫Romanomermis wuchangensis线粒体基因组全序列。序列的注释和分析结果表明,武昌罗索线虫线粒体基因组序列全长为14832bp,共有12个蛋白质基因、2个rRNA基因、23个tRNA基因和一段富含AT的D-loop区。与GenBank中现有的6种索科线虫序列对比,结果表明索科线虫线粒体基因组具有以下特点：①线粒体基因排列顺序各不相同;②部分线虫（包括种内）线粒体基因存在重复现象,且重复次数不同;③线粒体长度存在很大差异（14~26kb）。
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| 关 键 词： | 昆虫病原线虫 武昌罗索线虫 线粒体基因组 |
Sequencing and Analysis of Complete Mitochondrial Genome of Romanomermis wuchangensis |
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| HE Fang
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JIANG Ai-lan
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ZHAO Na-na
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LIU Hui
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WANG Guo-xiu.Sequencing and Analysis of Complete Mitochondrial Genome of Romanomermis wuchangensis[J].Chinese Journal of Biological Control,2010(1):66-72. |
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| Authors: | HE Fang JIANG Ai-lan ZHAO Na-na LIU Hui WANG Guo-xiu |
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| Institution: | HE Fang,JIANG Ai-lan,ZHAO Na-na,LIU Hui,WANG Guo-xiu(College of Life Sciences,Huazhong Normal University,Wuhan 430079,China) |
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| Abstract: | The mitochondrial genome of Romanomermis wuchangensis was sequenced by combining the rolling circle amplification with two-step long PCR.The results show that the mitochondrial genome of R.wuchangensis is 14832 base pairs in length.It contains 37 genes,including 12 protein-coding genes,2 ribosomal RNA and 23 transfer RNA,and also contains a putative control region and an AT-rich sequence.The characteristics of the mitochondrial genome of 7 species in Mermithidae were summarized.Firstly,gene orders of mitoch... |
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| Keywords: | entomogenous nematodes Romanomermis wuchangensis mitochondrial genome |
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