| 猪口蹄疫病毒多抗原表位的原核表达与纯化 |
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| 引用本文： | 杜以军,姜平,蒋文明,李玉峰.猪口蹄疫病毒多抗原表位的原核表达与纯化[J].畜牧与兽医,2006,38(10):1-3. |
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| 作者姓名： | 杜以军 姜平 蒋文明 李玉峰 |
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| 作者单位： | 南京农业大学农业部动物疫病诊断与免疫重点开放实验室,江苏,南京,210095 |
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| 基金项目： | 国家自然科学基金(B0270990)，新世纪优秀人才支持计划(NCET-04-0502) |
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| 摘 要： | 利用限制性酶切从重组质粒pShuttle-CMV-VP中得到猪O型口蹄疫病毒VP1(21-60)-(141-160)-(200-213)位氨基酸的基因。将此多抗原表位基因克隆至原核高效表达载体pET43.1 a(+),在E.coliBL21中用IPTG诱导表达了含有猪口蹄疫病毒多抗原表位的融合蛋白,并用镍柱亲和层析法获得了纯化蛋白。W estern-b lot结果表明融合蛋白可被猪O型口蹄疫病毒标准阳性血清所识别,从而为进一步研究FMDV多表位抗原的免疫特性和诊断方法奠定了基础。
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| 关 键 词： | 口蹄疫病毒 表位 原核表达 |
| 文章编号： | 0529-5130（2006）10-0001-03 |
| 收稿时间： | 02 22 2006 12:00AM |
| 修稿时间： | 07 10 2006 12:00AM |
Prokaryotic expression and purification of multiple antigen epitopes of swine foot-and-mouth disease virus |
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| DU Yi-jun,JIANG Ping,JIANG Wen-ming,LI Yu-feng.Prokaryotic expression and purification of multiple antigen epitopes of swine foot-and-mouth disease virus[J].Animal Husbandry & Veterinary Medicine,2006,38(10):1-3. |
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| Authors: | DU Yi-jun JIANG Ping JIANG Wen-ming LI Yu-feng |
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| Institution: | Key Laboratory of Animal Disease Diagnostic and Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China |
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| Abstract: | Amino acids(21-60)-(141-160)-(200-213)coding region of swine foot-and-mouth disease virus(FMDV)serotype O VP1 was successfully obtained from recombinant plasmid pShuttle-CMV-VP by restriction enzymes and cloned into prokaryotic expression(vector) pET43.1a( ).The fusion protein was highly expressed in E.coli BL21 induced by IPTG.It could be purified efficiently with Ni~ affinity chromatography column.Western-blot analysis showed that the fusion protein was able to react with swine polyclonal antibody against swine foot-and-mouth disease virus serotype O.It provided fundamental data and materials for the further investigation on immunogenicity of multiple epitopes vaccine of FMDV. |
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| Keywords: | foot-and-mouth disease virus epitope prokaryotic expression |
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