| 马铃薯X病毒外壳蛋白基因的克隆与系统进化分析 |
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| 引用本文： | 黄永会,朱英,刘永翔.马铃薯X病毒外壳蛋白基因的克隆与系统进化分析[J].安徽农业科学,2014(27):9283-9285,9420. |
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| 作者姓名： | 黄永会 朱英 刘永翔 |
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| 作者单位： | 贵州省生物技术研究所,贵州贵阳550006;贵州省农业生物技术重点实验室,贵州贵阳550006 |
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| 基金项目： | 贵州省农业科学院研究生科研创新基金项目“抗病毒转基因马铃薯的荧光原位杂交(FISH)研究”，贵州省科研机构创新能力建设专项“药用植物和作物资源研究与开发应用创新能力建设”[黔科合院所创能 |
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| 摘 要： | 从贵州威宁等地采集马铃薯病叶样本,采用试管捕捉反转录PCR（tube-capture-RT-PCR,TC-RT-PCR）的方法克隆出714 bp的PVX CP基因,编码237个氨基酸残基.系统进化分析表明,PVX CP基因可分为两大类群,推测分离物属于X3株系,并建议重型花叶也可作为PVX病叶样本.
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| 关 键 词： | 马铃薯X病毒 外壳蛋白 基因克隆 进化分析 |
Cloning and Phylogenetic Analysis of Potato Virus X CP Gene |
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| HUANG Yong-hui,ZHU Ying,LIU Yong-xiang.Cloning and Phylogenetic Analysis of Potato Virus X CP Gene[J].Journal of Anhui Agricultural Sciences,2014(27):9283-9285,9420. |
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| Authors: | HUANG Yong-hui ZHU Ying LIU Yong-xiang |
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| Institution: | HUANG Yong-hui;ZHU Ying;LIU Yong-xiang;Guizhou Institute of Biotechnology;Guizhou Key Lab for Agricultural Biotechnology; |
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| Abstract: | Potato leaves with PVX infection symptoms were collected from Guizhou Province including Weining County.A PVX CP gene of 714bp encoding 237 amino acid residues was cloned with the tube capture RT-PCR （TC-RT-PCR） method.Phylogenic analysis revealed that PVX CP genes can be divided into two groups,and the isolate was estimated to be X3 strain.Furthermore,We suggested that severe mosaic leaves could also be samples for PVX gene cloning. |
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| Keywords: | Potato virus X (PVX) Coat protein (CP) Gene cloning Phylogenetic analysis |
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