Sexing chick embryos: a rapid and simple protocol |
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| Authors: | Clinton M Haines L Belloir B McBride D |
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| Institution: | Roslin Institute (Edinburgh), Roslin, Midlothian EH25 9PS, Scotland. michael.clinton@BBSRC.AC.UK |
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| Abstract: | 1. Analysis of gene expression in the developing chick gonads requires the collection of male and female tissues from embryos between 3.5 d and 8.5 d of development. However, male and female chick embryos are indistinguishable by morphological examination before d 7.5 of development. 2. Sex identification of earlier embryos is only possible by molecular methods, which at present are laborious and time consuming. 3. We have devised a PCR-based sexing protocol which combines both sex specific and control reactions in a single tube assay. The assay is rapid and effective over a wide range of DNA concentrations and is tolerant of poor quality DNA. 4. Procedures are described for identifying the sex of individual embryos using either tissue samples or a small number of cells recovered from amniotic fluid. |
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