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Expression of Introduced Genes after Tuber Propagation of Transgenic Potato Plants
Authors:Marie-Paule  Ottaviani  Charlotte H  Hänisch ten Cate Lous  van Vloten-Doting
Institution:Institut für Angewandte Genetik, Universität Hannover, Herrenhäuser Straße 2, W-3000 Hannover 21, Germany
Abstract:The linkage relationship between eighteen isozyme loci and the morphological markers hypocotyl colour (R-r), monogerm character (M-m), pollen fertility (X) and stem fasciation (Verb.) are tested. Three linkage groups could be set up, involving all morphological marker loci and eight of the isozyme loci. Est-2, R-r, Fdp-2, Got2 and Icd-1 belong to linkage group I, linkage group II includes the loci Fas-fas M-m, Est-3 and Aco-1, linkage group III contains the loci X, Mdh-1 and Est-5. When analysing the inheritance of isozymes and RFLPs, deviations are usually found in some lines from the expected frequencies of a 3 : 1 or 1 : 2 : 1 segregation at single marker loci. In many cases these data can still be used for the estimation of recombination values with linked loci under the control of selection. Procedures to estimate linkage in such cases are given and applied to experimental data in Beta vulgaris.
Keywords:Beta vulgaris            isozyme marker  linkage  gametic selection  zygotic selection
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