| 草坪草的遗传转化研究进展 |
| |
| 引用本文： | 张俊卫,包满珠,孙振元.草坪草的遗传转化研究进展[J].林业科学研究,2003,16(1):87-94. |
| |
| 作者姓名： | 张俊卫 包满珠 孙振元 |
| |
| 作者单位： | 1. 华中农业大学园艺林学学院,武汉,430070
2. 中国林业科学研究院林业研究所,北京,100091 |
| |
| 基金项目： | 国家转基因专项基金项目，国家863计划资助项目，华中农业大学创新基金资助项目 |
| |
| 摘 要： | 概述了草坪草遗传转化的方法和发展趋势，重点介绍了草坪草的植株再生体系和草坪草的遗传转化方法的研究进展。通过愈伤组织培养，悬浮细胞培养，原生质体培养等手段，对早熟禾属，黑麦草属，结缕草属和剪股颖属的一些物种已建立了较为完善的植株再生体系。在这些再生体系的基础上，利用电激法，硅碳纤维法，PEG法和基因枪法在上述各属的一些物种上已成功地建立了遗传转化体系并获得了有一定应用前景的转基因植株。
|
| 关 键 词： | 草坪草 植株再生 遗传转化 |
| 文章编号： | 1001-1498(2003)01-0087-08 |
| 收稿时间： | 2002/3/10 0:00:00 |
Advances of Genetic Transformation of Turf Grass |
| |
| ZHANG Jun-wei,BAO Man-zhu and SUN Zhen-yuan.Advances of Genetic Transformation of Turf Grass[J].Forest Research,2003,16(1):87-94. |
| |
| Authors: | ZHANG Jun-wei BAO Man-zhu and SUN Zhen-yuan |
| |
| Institution: | College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan430070, Hubei, China;College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan430070, Hubei, China;Flower Center, CAF, Beijing100091, China |
| |
| Abstract: | The advances of techniques of plant regeneration and genetic transformation were reviewed in this paper. Plant regeneration system of the species of genus Lolium, Poa, Zoysia, Agrostis and Festuca were established by means of solid medium culture,suspension culture,and protoplast suspension culture. Based upon the established regeneration systems,the genetic transformation system of those species were also established by means of silicon carbide fibre,PEG,electroporation and microprojectile bombardment methods. |
| |
| Keywords: | turf grass species plant regeneration genetic transformation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《林业科学研究》浏览原始摘要信息 |
| 点击此处可从《林业科学研究》下载免费的PDF全文 |
|
