| 杂色鲍HSP90基因启动子的功能分析 |
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| 引用本文： | 张涛,张子平,贾锡伟,王淑红,王国栋,王艺磊.杂色鲍HSP90基因启动子的功能分析[J].水产学报,2017,41(4):490-497. |
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| 作者姓名： | 张涛 张子平 贾锡伟 王淑红 王国栋 王艺磊 |
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| 作者单位： | 1. 集美大学水产学院,农业部东海海水健康养殖重点实验室,福建厦门361021;2. 福建农林大学动物科学学院,福建福州,350002 |
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| 基金项目： | 国家自然科学基金（41176152）；集美大学创新团队基金（2010A001） |
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| 摘 要： | 为探索启动子在热休克蛋白90表达调控中的作用,本研究在课题组已有杂色鲍HSP90基因cDNA的基础上,通过Genome walking、Tail-PCR和常规PCR等技术克隆获得该基因的5′调控区序列。在翻译起始位点(ATG)和第一外显子(长度94 bp)之间有一个809bp的内含子,第一外显子之前的5′调控区共2800 bp,从预测的转录起始位点(A)起,共2811 bp。在转录起始位点(A)上游–30 bp处存在TATA box。潜在的转录因子结合位点包括ATF、TBP、Sp1、Oct-1、C/EBPalpha、NF-1、NF-κappa B、GATA-1、Sox-2等。CpG岛预测软件分析其含1个CpG岛,长度为131 bp。实验构建了8个启动子缺失片段的萤火虫荧光素酶表达载体,通过瞬时转染293T细胞并进行双荧光素酶报告基因活性检测,确定杂色鲍HSP90基因核心启动子区位于–98~83 bp。在–624~–539 bp,Oct-1、C/EBPalpha、NF-1这3个转录因子都起到一定的抑制作用。
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| 关 键 词： | 杂色鲍 HSP90 启动子 瞬时转染 功能分析 |
| 收稿时间： | 2016/3/15 0:00:00 |
| 修稿时间： | 2016/10/16 0:00:00 |
Functional analysis of the promoter in Haliotis diversicolor HSP90 gene |
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| ZHANG Tao,ZHANG Ziping,JIA Xiwei,WANG Shuhong,WANG Guodong and WANG Yilei.Functional analysis of the promoter in Haliotis diversicolor HSP90 gene[J].Journal of Fisheries of China,2017,41(4):490-497. |
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| Authors: | ZHANG Tao ZHANG Ziping JIA Xiwei WANG Shuhong WANG Guodong and WANG Yilei |
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| Institution: | Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, College of Fisheries, Jimei University, Xiamen 361021, China,College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China,Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, College of Fisheries, Jimei University, Xiamen 361021, China,Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, College of Fisheries, Jimei University, Xiamen 361021, China,Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, College of Fisheries, Jimei University, Xiamen 361021, China and Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, College of Fisheries, Jimei University, Xiamen 361021, China |
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| Abstract: | |
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| Keywords: | Haliotis diversicolor HSP90 promoter transient transfection functional analysis |
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