| 饲料中花生四烯酸对发育前期大菱鲆亲鱼性类固醇激素合成的影响 |
| |
| 引用本文： | 张圆琴,徐后国,曹林,卫育良,郑珂珂,梁萌青.饲料中花生四烯酸对发育前期大菱鲆亲鱼性类固醇激素合成的影响[J].水产学报,2017,41(4):588-601. |
| |
| 作者姓名： | 张圆琴 徐后国 曹林 卫育良 郑珂珂 梁萌青 |
| |
| 作者单位： | 1. 南京农业大学无锡渔业学院,江苏无锡214081;中国水产科学研究院黄海水产研究所,山东青岛266071;2. 中国水产科学研究院黄海水产研究所,山东青岛,266071;3. 南京农业大学无锡渔业学院,江苏无锡214081;中国水产科学研究院黄海水产研究所,山东青岛266071;青岛海洋科学与技术国家实验室,海洋渔业科学与食物产出过程功能实验室,山东青岛266071 |
| |
| 基金项目： | 国家自然科学基金（31402309）；中国博士后科学基金（2014M551991，2015T80763）；国家鲆鲽类产业技术体系（CARS-50-G08） |
| |
| 摘 要： | 为研究饲料中不同水平的花生四烯酸(arachidonic acid,ARA)对发育前期的大菱鲆亲鱼性类固醇激素合成量及合成过程的影响,配制3种等氮等脂(脂肪含量13%)的实验饲料,分别含有不同梯度水平的花生四烯酸:0.72%(不添加花生四烯酸精制油的对照组,C),5.63%(添加低水平ARA的处理组,ARA-L)及15.03%(添加高水平ARA的处理组,ARA-H)(各数值均为占总脂肪酸的比例)。每组饲料投喂3个实验桶,每桶放25尾3龄大菱鲆亲鱼(雌雄比例约为1:1)。养殖实验在室内流水系统内进行,每天饱食投喂2次,养殖周期为5个月。养殖结束后,分别取性腺发育前期的雌雄鱼测血清雌二醇和睾酮的含量并检测性腺中性类固醇激素合成相关蛋白质的基因表达量。结果显示:与对照组相比,饲料中高水平的花生四烯酸显著降低了雌鱼血清中雌二醇的含量,而雄鱼血清中睾酮的含量在低水平花生四烯酸处理组显著降低。在卵巢中,饲料中高水平的花生四烯酸显著降低了促卵泡激素受体mRNA的表达量,但是饲料中低水平的花生四烯酸显著提高了17α羟化酶的mRNA表达量。在精巢中,饲料中低水平的花生四烯酸显著降低了固醇合成急性调节蛋白以及17α羟化酶的mRNA表达量,但显著升高了芳香化酶的mRNA表达量。饲料中花生四烯酸提高了性腺、肝脏和肌肉组织中花生四烯酸的含量,但降低了二十碳五烯酸(EPA)的含量,卵巢中的花生四烯酸累积量高于精巢。综上所述,饲料中添加一定量的花生四烯酸抑制了发育前期大菱鲆亲鱼雌二醇和睾酮的合成,在卵巢中,这种抑制作用可能是通过抑制促卵泡激素受体的表达来实现,而在精巢中,可能是通过抑制固醇合成急性调节蛋白以及17α羟化酶来实现。
|
| 关 键 词： | 大菱鲆 亲鱼 饲料 花生四烯酸 性类固醇激素合成 基因表达 |
| 收稿时间： | 2016/7/8 0:00:00 |
| 修稿时间： | 2016/9/20 0:00:00 |
Effects of dietary arachidonic acid on the sex steroid hormone synthesis in turbot broodstock before maturation |
| |
| ZHANG Yuanqin,XU Houguo,CAO Lin,WEI Yuliang,ZHENG Keke and LIANG Mengqing.Effects of dietary arachidonic acid on the sex steroid hormone synthesis in turbot broodstock before maturation[J].Journal of Fisheries of China,2017,41(4):588-601. |
| |
| Authors: | ZHANG Yuanqin XU Houguo CAO Lin WEI Yuliang ZHENG Keke and LIANG Mengqing |
| |
| Institution: | Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China,Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China and Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China |
| |
| Abstract: | To investigate the effects of dietary arachidonic acid (ARA) on the sex steroid hormone production and the gene expressions of the key proteins in steroidogenesis in gonads of turbot Scophthalmus maximus broodstocks,three isonitrogenous and isolipidic (lipid content 13％) experimental diets were formulated to contain different ARA levels:the control diet without ARA supplementation (C,0.72％ ARA of total fatty acids) and two diets with low (5.63％ of total fatty acids,ARA-L) or high ARA (15.03％ of total fatty acids,ARA-H) level.The experimental diets were randomly assigned to 9 tanks of 3-year-old turbot (25 fish in each tank).Fish were reared in an indoor flowing seawater system and fed to apparent satiation twice daily.At the end of the feeding trial,sex steroid hormone production was assayed and gonad samples from females and males were collected to determine the gene expressions of sex steroid-synthesizing proteins.The results showed that compared to the control group,ARA-H significantly reduced the estradiol production in females but in males ARA-L significantly reduced the testosterone production.In ovaries,ARA-H significantly reduced the mRNA expression of follicle stimulating hormone receptor,but ARA-L significantly increased the mRNA expression of 17α-hydroxylase.In testes,ARA-L significantly reduced the mRNA expression of steroidogenic acute regulatory protein and 17α-hydroxylase,but significantly increased the mRNA expression of aromatase.Dietary ARA supplementation increased the ARA accumulation but decreased the EPA accumulation in fish tissues.The ARA concentration in ovaries was higher than that in testes.In conclusion,certain content of dietary ARA inhibited the estradiol and testosterone production in immature turbot broodstock.In ovaries,this inhibition may be attributed to the inhibition on follicle stimulating hormone receptor,but in testes it may be attributed to the inhibition on steroidogenic acute regulatory protein and 17a-hydroxylase. |
| |
| Keywords: | Scophthalmus maximus broodstock diet arachidonic acid sex steroid hormone synthesis gene expression |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《水产学报》浏览原始摘要信息 |
| 点击此处可从《水产学报》下载免费的PDF全文 |
|
