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猕猴桃蛋白酶提取的工艺技术研究
引用本文:左玉萍,冯成利,党蕊叶.猕猴桃蛋白酶提取的工艺技术研究[J].西北农业学报,2006,15(6):127-129.
作者姓名:左玉萍  冯成利  党蕊叶
作者单位:陕西省动物研究所生化室,陕西西安,710032
摘    要:采用盐析沉淀法,配合等电点沉淀法,对猕猴桃蛋白酶提取的工艺技术进行了研究。初步选定的工艺条件为:果汁与水的比例为1∶1,食盐水浓度为22.5%~25%(W/V),pH值范围在4.0~4.8间,低温沉淀8~10 h。粗酶的平均提取率约为0.765%。

关 键 词:猕猴桃蛋白酶  提取  工艺技术
文章编号:1004-1389(2006)06-0127-03
收稿时间:2006-03-07
修稿时间:2006-04-26

Extraction Technology of Actinidin in Actinidia chinese
ZUO Yu-ping,FENG Cheng-li and DANG Rui-ye.Extraction Technology of Actinidin in Actinidia chinese[J].Acta Agriculturae Boreali-occidentalis Sinica,2006,15(6):127-129.
Authors:ZUO Yu-ping  FENG Cheng-li and DANG Rui-ye
Institution:Shaanxi Institute of Zoology, Xi''an 710032, China;Shaanxi Institute of Zoology, Xi''an 710032, China;Shaanxi Institute of Zoology, Xi''an 710032, China
Abstract:This paper reports a kind of extraction technology of Actinidin by adoping the salting out and isoelectric precipitation method, in order to meet the needs of market.The optimizing extraction condition are: the ratio of material to water is 1:1;concentration of salt water is 22.5%~25%(W/V);pH range is 4.0~4.8; time of sediment is about 8~10 h.The average extraction ratio of crude enzyme is 0.765%.
Keywords:Actinidin  Extraction  Technology
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