| 子房切片技术获得百合杂交种 |
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| 引用本文： | 柴卫淑,谭学林,熊丽,屈云慧.子房切片技术获得百合杂交种[J].黑龙江农业科学,2007(3):67-69. |
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| 作者姓名： | 柴卫淑 谭学林 熊丽 屈云慧 |
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| 作者单位： | 1. 云南农业大学生物技术与应用学院,昆明,650201
2. 云南省农科院,昆明,650205 |
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| 摘 要： | 以不同杂种系的百合品种进行远缘杂交,采收授粉后5～20 d的子房,用子房切片培养方法挽救杂种胚,培育杂种.试验结果表明:百合远缘杂交中,采用子房切片方法挽救杂种胚,培育出杂种苗的关键是合适的胚龄:7～12 d的胚龄比较合适,这一胚龄段中,胚龄越小,胚萌发需要的时间越长.通过品种远缘杂交,培育出了少量的百合远缘杂种.
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| 关 键 词： | 百合 杂交 胚挽救 子房切片 子房 切片 技术获得 百合 杂交种 Technology Slice Ovary Interspecific Hybridization Derived 远缘杂种 时间 胚萌发 比较 胚龄 杂种苗 培养方法 结果 试验 杂种胚 |
| 文章编号： | 1002-2767（2007）03-0067-03 |
| 收稿时间： | 2006-11-15 |
| 修稿时间： | 2006年11月15 |
Hybrids Derived from Interspecific Hybridization in Lilium through Ovary Slice Technology |
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| CHAI Wei-shu,TAN Xue-lin,XIONG Li,QU Yun-hui.Hybrids Derived from Interspecific Hybridization in Lilium through Ovary Slice Technology[J].Heilongjiang Agricultural Science,2007(3):67-69. |
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| Authors: | CHAI Wei-shu TAN Xue-lin XIONG Li QU Yun-hui |
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| Institution: | 1. Agronomy and Biotechnology College, Yunnan Agricultural University, Kunming 650201;2. Yunnan Academy of Agricultural Sciences, Kunming 650205 |
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| Abstract: | Interspecific crosses were made to produce hybrids in Lilium, and the hybrid embryos were rescued by using the ovary slice culture after 5-20 days of pollination. The results showed that embryo age was a key factor to success in the ovary slice culture in Lilium interspecific hybridization. If embryo age was younger, more days were required for embryo germination. We got a few hybrids. |
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| Keywords: | lily hybridization embryo rescue ovary slice |
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