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Effect of Seminal Plasma Components on the Quality of Fresh and Cryopreserved Stallion Semen
Institution:1. College of Veterinary Medicine and Animal Science, CES University, Medellín, Colombia;2. College of Sciences, School of Chemistry, Universidad Nacional de Colombia, Medellín, Colombia;3. Department of Animal Production, Universidad Nacional de Colombia, Medellín, Colombia;1. Institute for Animal Breeding and Genetics, University of Veterinary Medicine Hannover (Foundation), Hannover, Germany;2. Unit of Reproductive Medicine—Clinic for Horses, University of Veterinary Medicine Hannover (Foundation), Hannover, Germany;3. Lower Saxon National Stud Celle, Celle, Germany;1. Clinic of Reproductive Medicine, Vetsuisse-Faculty University of Zürich, Switzerland;2. Swiss Institute of Equine Medicine ISME, Agroscope and University of Bern, Avenches, Switzerland;3. Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Faculty of Veterinary Medicine, Justus-Liebig-University, Giessen, Germany;1. Division for Obstetrics and Gynaecology, Department for Small Animals and Horses, Vetmeduni Vienna, Vienna, Austria;2. Centre for Artificial Insemination and Embryo Transfer, Department for Small Animals and Horses, Vetmeduni Vienna, 1210 Vienna, Austria;1. Clinic for Horses—Unit for Reproductive Medicine, University of Veterinary Medicine Hanover, Hanover, Germany;2. Veterinary Clinic Domaene Karthaus, Duelmen, Germany;3. Hanoverian National Stud Celle, Celle, Germany;4. Swiss Institute of Equine Medicine, Agroscope and University of Berne, Avenches, Switzerland;1. Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, Spain;2. Department of Clinical and Experimental Medicine (IKE), Linköping University, Sweden
Abstract:The importance of seminal plasma (SP) components for stallion semen quality and freezability is little known. This study aimed to evaluate the relationship between SP components and fresh/cryopreserved stallion semen quality. Semen of 30 stallions was collected, and then, SP was recovered and lyophilized. Total protein (TP), vitamin C (CVIT), vitamin E (EVIT), vitamin A (AVIT), iron (Fe), copper (Cu), magnesium, and zinc (Zn) in SP were assessed. Sperm was frozen in an extender supplemented with lyophilized SP. In fresh semen motility, abnormal morphology (AM), sperm vitality (SV), and plasma membrane integrity (PMI) were evaluated. In post-thaw semen, additionally, total motility (TM), progressive motility (PM), straight line velocity (VSL), curvilinear velocity (VCL), average path velocity (VAP), amplitude of lateral head displacement (ALH), and beat cross-frequency (BCF) were assessed. Levels of component of SP were established by a distribution analysis. Generalized linear models were fitted. Comparisons of means were done with Tukey's test. Correlation and regression analyses were performed. Vitamins and ions were found to be related to fresh semen quality. For post-thaw sperm, medium TP showed higher semen quality. Negative regression and correlation coefficients between CVIT and all post-thaw semen parameters were found. Low EVIT yielded the lowest PM, VSL, and VAP values, while a high level of AVIT yielded the best results for sperm quality. A high level of Cu yielded higher results for TM, PM, VCL, and ALH. Moreover, a negative correlation was found between Zn, SV, and PMI. In conclusion, SP composition influences fresh and post-thaw stallion semen quality.
Keywords:Seminal plasma  Component  Stallion  Sperm quality  Cryopreservation
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