The Effect of Trehalose Supplementation of INRA-82 Extender on Quality and Fertility of Cooled and Frozen-Thawed Stallion Spermatozoa |
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| Institution: | 1. Department of Artificial Insemination and Embryo Transfer, Animal Reproduction Research Institute, Agriculture Research Center, Giza, Egypt;2. Department of Animal Reproduction and A.I., National Research Center, Dokki, Giza, Egypt;1. Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Razi Vaccine and Serum Research Institute (RVSRI), Karaj, Iran;3. Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz, Iran;4. Centre for Animal Science Studies (CECA/ICETA), Campus Agrario de Vairão, University of Porto, Vairão, Portugal;1. Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA;2. Department of Anatomy, Cell Biology, and Physiology, School of Veterinary Medicine, University of California, Davis, CA;1. Department of Animal Science, University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran;2. Department of Clinical Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University, Multan, Pakistan;1. Department of Biomedical Sciences, Colorado State University, Equine Reproduction Laboratory, 3101 Rampart Road, Fort Collins, CO, 80521, USA;2. Graduate Program in Animal Medicine (Equine), Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil;1. College of Veterinary and Animal Sciences, RAJUVAS, Bikaner, Rajasthan, India;2. Equine Production Campus, ICAR-NRCE, Bikaner, Rajasthan, 334001, India;3. ICAR-National Research Centre on Equines, Hisar, Haryana, 225001, India;1. ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana, India;2. Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India |
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| Abstract: | Stallion semen cryopreservation is often associated with poor post-thaw sperm quality. Sugars act as nonpermeating cryoprotectants. The aim of the present study was to evaluate the cryoprotective effect of trehalose on stallion sperm quality and field fertility rates subjected to cooling and freeze–thaw process. Semen samples were collected from six Arabian stallions, divided into five different treatments in a final concentration of 100 × 106 sperm/mL by using INRA-82 extender containing 0, 25, 50, 100, and 200 mM of trehalose then subjected to both cold storage and cryopreservation. Sperm motility, acrosome, plasmatic membrane, and DNA integrity were analyzed, and 57 mares were used to evaluate the field fertility of chilled and frozen-thawed semen. Results showed that the extender containing 100 mM trehalose only increased the functional acrosomal, plasma membrane, and DNA integrities. The inclusion of 50 mM trehalose in semen extender resulted in significantly (P < .05) increased post-thaw total motility compared to the control group, and chilled semen achieved higher pregnancy rates compared to the frozen-thawed one. Pregnancy rate of mares inseminated with frozen-thawed semen (P < .05; 46.15% vs. 36.36%, respectively) was lower than those inseminated with chilled semen (76.47% vs. 68.75%, respectively) but higher than control. In conclusion, addition of 50 mM trehalose yielded the highest quality stallion semen after cooling and post-thawing in terms of motility, integrities of acrosome, membrane, and DNA as well as improved field fertility. |
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| Keywords: | Semen extender Trehalose Chilled Cryopreservation Stallion |
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