Accuracy and Precision of Mini-FLOTAC and McMaster Techniques for Determining Equine Strongyle Egg Counts |
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| Institution: | 1. Department of Parasitology and Invasive Diseases, University of Life Sciences in Lublin,12 Akademicka Street, 20-950, Lublin Poland;2. Department of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences,30 G??boka street, 20-612 Lublin, Poland;3. Department of Small Ruminants Breeding and Agriculture Advisor, University of Life Sciences in Lublin,13 Akademicka street, 20-950 Lublin, Poland;1. Comparative Tropical Medicine and Parasitology, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität, Leopoldstr. 5, D-80802 Munich, Germany;2. IAgriBio, Department of Animal, Plant and Soil Science, La Trobe University, Bundoora, Victoria 3086, Australia and Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, Garscube Campus, Bearsden Road, Glasgow G61 1QH, UK;1. Moredun Research Institute, Pentlands Science Park, Bush Loan, Penicuik EH26 0PZ, UK;2. Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy;3. Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Belgium;1. M.H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA;2. MEP Equine Solutions, 3905 English Oak Circle, Lexington, KY, 40514, USA;1. M.H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY 40508, USA;2. MEP Equine Solutions, 3905 English Oak Circle, Lexington, KY 40514, USA;1. MEP Equine Solutions, 3905 English Oak Circle, Lexington, KY 40514, USA;2. M.H. Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington, KY, USA;3. Department of Molecular & Cellular Biochemistry, University of Kentucky, Lexington, KY, USA |
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| Abstract: | The use of fecal egg count techniques to indirectly assess intestinal parasite burdens and determine anthelmintic efficacy is common in parasitological research and veterinary practice. The McMaster method is one of the most widely used techniques in veterinary practice, but recently, the Mini-FLOTAC technique has been introduced as a possible alternative. Studies comparing the two methods in precision and accuracy are needed. This study aimed at evaluating the Mini-FLOTAC technique for determining equine strongyle egg counts through a two-part procedure. First, a set of fecal counts was executed using both methods. Next, blind counts were performed on spiked fecal samples with true egg counts at 0, 5, 50, 500, and 1,000 eggs per gram. All counts were performed in triplicates, and each sample was counted using both methods. Mini-FLOTAC and McMaster had 83.2% and 53.7% precision, respectively. The accuracy was found to be 42.6% and 23.5% for Mini-FLOTAC and McMaster, respectively. In conclusion, this study found that Mini-FLOTAC exhibited both higher precision and accuracy than the McMaster technique and appears to be a more reliable alternative. Using a more precise egg-counting method can help assure that changes in egg counts before and after treatment reflect a genuine reduction and are not due to chance variability. |
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| Keywords: | Horse Fecal egg count McMaster Strongyle Mini-FLOTAC |
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