The Effects of an Oxygen Scavenger and Coconut Water on Equine Sperm Cryopreservation |
| |
| Institution: | 1. Department of Population Health and Reproduction, School of Veterinary Medicine, University of California, Davis, CA;2. Department of Anatomy, Cell Biology, and Physiology, School of Veterinary Medicine, University of California, Davis, CA;1. Department of Zootechnic Sciences, Coimbra College of Agriculture, Polytechnic Institute of Coimbra, Bencanta, 3040-316 Coimbra, Portugal;2. CERNAS – Natural Resources, Environment and Society Centre-ESAC, Bencanta, 3040-316 Coimbra, Portugal;3. Universidade de Lisboa, Faculdade de Ciências, Centro de Biologia Animal, Campo Grande, 1749-016 Lisboa, Portugal;4. Associação Internacional de Criadores do Cavalo Ibérico de Tipo Primitivo-Sorraias, Barbacena, Portugal;5. Centro de Estudos em Ciência Animal (CECA), Instituto de Ciências Biomédicas Abel Salazar (ICBAS), University of Porto, Oporto, Portugal;1. Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran;2. Department of Clinical Science, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran;1. AUB-Instituto Nazionale Fecondazione Artificiale, University of Bologna, Granarolo dell''Emilia, Bologna, Italy;2. Department of Veterinary Medical Sciences, University of Bologna, Ozzano Emilia, Bologna, Italy;3. Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, TX;1. Department of Animal Physiology, Federal University of Agriculture Abeokuta, P.M.B 2240 Abeokuta, Ogun State, Nigeria;2. Department of Animal Breeding and Genetics, Federal University of Agriculture Abeokuta, P.M.B 2240 Abeokuta, Ogun State, Nigeria;1. Clinic of Reproductive Medicine, Vetsuisse-Faculty University of Zürich, Switzerland;2. Swiss Institute of Equine Medicine ISME, Agroscope and University of Bern, Avenches, Switzerland;3. Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Faculty of Veterinary Medicine, Justus-Liebig-University, Giessen, Germany |
| |
| Abstract: | Alternative sources of lipoproteins in semen extenders could replace animal by-products. We hypothesized that: (1) post-thaw semen parameters and fertility would not be different in coconut water (CW)–treated samples compared with egg yolk (EY)–treated samples and (2) the use of an oxygen scavenger (Oxyrase) would improve post-thaw sperm motility and membrane integrity and decrease lipid peroxidation. Experiment 1: three ejaculates each from five stallions were split into four treatments: EY, CW, egg yolk with Oxyrase, and coconut water with Oxyrase. Computer-assisted sperm analysis measured progressive and total motility, velocity, and linearity. Membrane integrity, apoptosis, and lipid peroxidation were evaluated using propidium iodide, annexin, and BODIPY fluorescent probes, respectively. Samples were cryopreserved, stored in liquid nitrogen, and then thawed to 37°C and analyzed again. Experiment 2: one ejaculate was divided into two aliquots and cryopreserved using either CW or EY. In a crossover design, 12 mares were bred on two consecutive cycles with either EY or CW. Pregnancy evaluations were at 14-day gestation. No differences were detected in sperm parameters between CW and EY (P > .05). Oxyrase did not improve sperm motility parameters in post-thaw samples, nor did it show protective effects for viability or against membrane damage (P > .05). More mares became pregnant using CW than EY (11/12 vs. 6/12, respectively; P = .013). Use of CW is a viable alternative to animal-based products in the cryopreservation of stallion semen. |
| |
| Keywords: | Coconut water Egg yolk Sperm cryopreservation Stallion sperm Oxyrase |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
