Morphology and Morphometry of Seminiferous Tubules in Crioulo Horses |
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| Institution: | 1. Department of Veterinary Medicine, Federal University of Santa Catarina, Curitibanos, SC, Brazil;2. Department of Veterinary Medicine, Laboratory of Animal Embryology, Federal University of Santa Maria, Santa Maria, RS, Brazil;3. Department of Veterinary Medicine, Laboratory of Assisted Reproduction, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil;4. Department of Veterinary Medicine, University of Cuiabá, Cuiabá, MT, Brazil;5. Department of Veterinary Medicine, University of Alfenas, Alfenas, MG, Brazil;1. Department of Anesthesiology, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27159-1009, USA;2. Department of Cardiology, Jinan Central Hospital, Affiliated with Shandong University, 105 Jiefang Road, Jinan, 250013, China;3. Hypertension and Vascular Disease Center, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA;4. Office of Women in Medicine and Science, Wake Forest School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA;1. Department of Cellular Biology and Histology, IMIB, Medical School, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, Murcia, Spain;2. Reproductive Biology Unit, Department of Biology, Faculty of Sciences, University of Girona, Campus de Montilivi, Girona, Spain;3. Department of Statistics, Medical School, University of Murcia, Campus de Espinardo, Murcia, Spain;1. Department of Pediatric Surgery, Ankara University School of Medicine, Ankara, Turkey;2. Department of Histology, Ankara University School of Medicine, Ankara, Turkey;3. Department of Immunology, Gazi University Faculty of Medicine, Ankara, Turkey;4. Department of Chemistry, Middle East Technical University, Ankara, Turkey;1. University of the Azores, Department of Biology, Ponta Delgada, Azores, Portugal;2. CIBIO Azores, Center for Research on Biodiversity and Genetic Resources, InBIO Associated Laboratory, University of the Azores, Ponta Delgada, Azores, Portugal;3. CE3C, Center for Ecology, Evolution and Environmental Changes, and Azorean Biodiversity Group, University of the Azores, 9501-801 Ponta Delgada, Portugal;4. CVARG, Center for Volcanology and Evaluation of Geological Risks, Ponta Delgada, Portugal;1. Department of Anatomy and Physiology, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, Pretoria, 0110, South Africa;2. Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110, Pretoria, South Africa;3. Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, Pretoria, 0110, South Africa;4. Department of Veterinary Anatomy, Faculty of Veterinary Medicine, PMB 1069 University of Maiduguri, Maiduguri, Nigeria;5. Department of Anatomy and Embryology, Faculty of Veterinary Medicine, South Valley University, Qena, 83523, Egypt;6. Department of Basic Science, University of West Kordofan, West Kordofan State, Sudan |
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| Abstract: | This study aimed to assess the biometrics of the testes and the morphology of the seminiferous tubules of Crioulo horses. We studied 10 sexually mature stallions (3–6 years of age). After orchiectomy, testes were perfused with Karnovsky's solution and then embedded in glycol methacrylate. Testis sections (4 μm) were cut and stained with toluidine blue and a solution of 1% sodium borate. The histological images were digitized, and the morphometric analysis was performed using ImageJ software. The average weight of the stallions was 377.5 kg, and the average weight of both testicles was 162.9 g. The percentage of testicular parenchyma occupied by the seminiferous tubules and the intertubular tissue was 77.97% ± 6.34% and 22.03% ± 6.34%, respectively. The average tubular diameter was 205.00 ± 36.91 μm, whereas the average height of the seminiferous epithelium was 70.56 ± 2.82 μm. Average tubular length per testicle and average tubular length per gram of testicle were 4,085.10 ± 1,170.68 m and 26.09 ± 10.63 m/g, respectively. The characteristics of the eight stages of the seminiferous epithelium cycle were similar to those reported in other horse breeds. We conclude that the morphometry of the seminiferous tubules of Crioulo horse resembles what has been reported in other horse breeds. The volumetric proportion of the seminiferous tubules and the Leydig cells of the Crioulo horse is one of the highest ever reported for stallions. |
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| Keywords: | Equine Testis Spermatogenesis |
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