| 西藏蜂胶抑菌活性及对金钗石斛茄病镰刀菌抑菌机理探究 |
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| 引用本文： | 王芳,张硕,杜琳,唐榆婷,覃悦,王岚.西藏蜂胶抑菌活性及对金钗石斛茄病镰刀菌抑菌机理探究[J].安徽农业大学学报,2023,50(5):847-854. |
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| 作者姓名： | 王芳 张硕 杜琳 唐榆婷 覃悦 王岚 |
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| 作者单位： | 成都师范学院化学与生命科学学院，成都 611130; 成都师范学院特色园艺生物资源开发与利用四川省高等学校重点实验室，成都 611130 |
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| 基金项目： | 成都师范学院校级项目（CS18ZD02）和教育部大学生创新创业训练计划支持项目（S202114389186）共同资助。 |
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| 摘 要： | 为探究西藏蜂胶的抑菌活性及对金钗石斛茄病镰刀菌作用机理，以2种细菌3种真菌为供试菌，采用牛津杯法、菌饼法、最低抑菌浓度(MIC)和最低杀菌浓度(MBC)对西藏蜂胶的抑菌活性进行评价。通过研究西藏蜂胶处理后金钗石斛茄病镰刀菌菌丝形态、生长速率、细胞膜通透性及呼吸强度探究其抑菌机理。结果表明：(1)西藏蜂胶具有广谱抑菌性，其抑菌能力显著强于市售蜂胶。(2)以80%乙醇提取剂在pH为6的条件下提取的西藏蜂胶对5种供试菌抑菌效果最佳。(3)西藏蜂胶浓度为10 mg·mL-1时对金钗石斛茄病镰刀菌抑制率达89%，抑菌中浓度(EC50)为2.552mg·mL-1；菌丝体细胞形态由饱满破裂到干瘪，细胞膜通透性增强，内容物泄漏；呼吸强度增大，能量代谢受阻，抑制菌丝体生长。综上所述，西藏蜂胶为天然广谱抑菌剂，利用其防治茄病镰刀菌所引起的植物性病害具有良好的开发前景及研究潜力。
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| 关 键 词： | 西藏蜂胶 抑菌活性 金钗石斛 茄病镰刀菌 抑菌机理 |
Study on the antibacterial activity of Tibetan propolis and the inhibitory mechanism of Haematonectria haematococca of Dendrobium nobile |
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| WANG Fang,ZHANG Shuo,DU Lin,TANG Yuting,QIN Yue,WANG Lan.Study on the antibacterial activity of Tibetan propolis and the inhibitory mechanism of Haematonectria haematococca of Dendrobium nobile[J].Journal of Anhui Agricultural University,2023,50(5):847-854. |
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| Authors: | WANG Fang ZHANG Shuo DU Lin TANG Yuting QIN Yue WANG Lan |
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| Institution: | College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu 611130; Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, Chengdu Normal University, Chengdu 611130 |
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| Abstract: | The purpose of this study is to look into the antibacterial activity of Tibetan propolis and the inhibitory mechanism in Haematonectria haematococca of Dendrobium nobile. To evaluate the antibacterial activity of Tibetan propolis, two types of bacteria and three types of fungi were studied with the Oxford cup method, the fungus cake method, the minimum inhibitory concentration (MIC) and the minimum bactericidal/fungicidal concentration (MBC), respectively. The hyphal morphology, growth rate, cell membrane permeability, and respiration intensity of H. haematococca after treatment with Tibetan propolis were used to investigate the antifungal mechanism. The results showed that: (1) Tibetan propolis that had broad-spectrum antibacterial activity was significantly stronger than that of commercially available propolis. (2) On the five tested strains, Tibetan propolis extracted with 80% ethanol at pH 6 had the best antibacterial activity. (3) At a concentration of 10 mg·mL-1 of Tibetan propolis, the mycelial growth inhibition rate was 89%, and the EC50 was 2.552 mg·mL-1; under these conditions, the morphology of the mycelium cells changed from full rupture to shriveling, which increased the cell membrane permeability and content leakage. Furthermore, the respiration intensity increased, which blocked the energy metabolism and inhibited the mycelium growth. In summary, Tibetan propolis is a natural broad-spectrum antibacterial agent with promising the development and research potential for controlling plant diseases caused by H. haematococca. |
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