Evaluation of biomarkers for osteoarthritis caused by fragmented medial coronoid process in dogs |
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| Institution: | 1. Tierklinik Dresdner Heide, Fischhausstrasse 5, 01099 Dresden, Germany;2. Institute of Veterinary Biochemistry, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany;1. Servicio Microbiología Clínica y Enfermedades Infecciosas, Hospital General Universitario Gregorio Marañón, Madrid, Spain;2. Instituto de Investigación Sanitaria Gregorio Marañón, Madrid, Spain;3. Centro de Vigilancia Sanitaria Veterinaria (VISAVET), Universidad Complutense, Madrid, Spain;4. CEI Campus Moncloa, UCM-UPM, Madrid, Spain;5. CIBER Enfermedades Respiratorias, CIBERES, Spain;6. Departamento de Medicina, Facultad de Medicina, Universidad Complutense, Madrid, Spain;7. Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain;1. Faculdade de Ciências Agrárias e Veterinárias, UNESP/CPPAR, Via de acesso prof. Paulo Donatto Castellani, Jaboticabal, São Paulo, Brazil;2. Ouro Fino Agronegócios, Rodovia Anhanguera, Km 298, Cravinhos, São Paulo, Brazil. CEP: 14140-000;1. Department of Small Animals, Universidade Federal de Santa Maria, Hospital Veterinário Universitário, UFSM. Avenida Roraima n° 1000, Camobi, Santa Maria, RS, CEP 97105-900, Brazil;2. Department of of Animal Science, Universidade do Estado de Santa Catarina, Rua Beloni Trombeta Zanin, 680-E. Bairro Santo Antônio, Chapecó, Santa Catarina, CEP 89815-630, Brazil;3. Department of Chemistry, Universidade Federal de Santa Maria, Avenida Roraima, Prédio 18 – Cidade Universitária; Faixa de Camobi, KM 9; Bairro Camobi; Santa Maria/RS, CEP 97105-900, Brazil;4. Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Porto Alegre, Rio Grande do Sul, CEP 90035-003, Brazil;5. Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, Centro de Ciências Rurais, Campus Universitário, Camobi – Santa Maria, Rio Grande do Sul, CEP 97105-000, Brazil;6. Department of Pathology, Universidade Federal de Santa Maria, Hospital Veterinário Universitário, prédio 97B, UFSM – Avenida Roraima, n° 1000, Camobi, Santa Maria, Rio Grande do Sul, CEP 97105-900, Brazil;1. Danke Veterinary Clinic, Laprida 1502, Postal Code 1832 Lomas de Zamora Municipality, Buenos Aires Province, Argentina;2. Machipin Veterinary Clinic, Av. San Martín 1709, Postal Code 1846 Adrogué, Buenos Aires, Argentina |
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| Abstract: | The aim of the present work was to evaluate whether concentrations of the carboxy-terminal cross-linked fragment of type II collagen (CTX-II), the activities of matrix metalloproteinase-2 and -9 (MMP-2/-9) and Myeloperoxidase (MPO) in canine synovial fluids (SF) can reflect structural alterations of articular cartilage in dogs with fragmented medial coronoid process (FMCP). Elbow joints with FMCP underwent radiographic and arthroscopic examination. Commercially available assays were used to analyze SF for CTX-II concentration and MMP-2/-9 activity. MPO activity was measured by o-dianisidine-assay. The MMPs were further evaluated by zymography. CTX-II concentration and MMP-2 activity showed age-dependent trends in controls. Increased enzyme activities of MPO and MMP-2/-9 were found in diseased dogs. MMP-9activity seems suitable to underline the subjective assessment of the degree of cartilage damage. These initial data of the study suggest that MPO and MMP-2/9 may be used as objective biomarkers in the diagnosis of canine osteoarthritis due to FMCP. |
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