Development and validation of a 4-plex antibody assay for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, and porcine reproductive and respiratory syndrome virus types 1 and 2 |
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| Institution: | 1. Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 1600 S. 16th Street, Ames, IA 50011, USA;2. Laboratório de Pesquisa em Virologia Animal, Departamento de Medicina Veterinária Preventiva, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil;3. Department of Diagnostic Medicine and Pathobiology, Kansas State University, Manhattan, KS 66506, USA;4. Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061, USA;5. Institute of Preventive Veterinary Medicine & Key Laboratory of Animal Virology of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China;6. The Roslin Institute and The Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian EH25 9RG, UK;1. Laboratory of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, Republic of Korea;2. Bioindustrial Process Reasearch Center and AI Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, Republic of Korea;3. Infection Control Material Research Center and AI Control Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 580-185, Republic of Korea;4. Chonnam National University Veterinary Teaching Hospital, Gwangju 500-757, Republic of Korea;5. Division of Applied Life Science, EB-NCR, Institute of Agriculture and Life Science, Graduate School of Gyeongsang National University, Jinju 660-701, Republic of Korea;1. Department of Small Animals, Universidade Federal de Santa Maria, Hospital Veterinário Universitário, UFSM. Avenida Roraima n° 1000, Camobi, Santa Maria, RS, CEP 97105-900, Brazil;2. Department of of Animal Science, Universidade do Estado de Santa Catarina, Rua Beloni Trombeta Zanin, 680-E. Bairro Santo Antônio, Chapecó, Santa Catarina, CEP 89815-630, Brazil;3. Department of Chemistry, Universidade Federal de Santa Maria, Avenida Roraima, Prédio 18 – Cidade Universitária; Faixa de Camobi, KM 9; Bairro Camobi; Santa Maria/RS, CEP 97105-900, Brazil;4. Department of Biochemistry, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2600, Porto Alegre, Rio Grande do Sul, CEP 90035-003, Brazil;5. Department of Preventive Veterinary Medicine, Universidade Federal de Santa Maria, Centro de Ciências Rurais, Campus Universitário, Camobi – Santa Maria, Rio Grande do Sul, CEP 97105-000, Brazil;6. Department of Pathology, Universidade Federal de Santa Maria, Hospital Veterinário Universitário, prédio 97B, UFSM – Avenida Roraima, n° 1000, Camobi, Santa Maria, Rio Grande do Sul, CEP 97105-900, Brazil;1. Department of Organic and Inorganic Chemistry, QUOREX Research Group, Faculty of Science-UEX, Badajoz, Spain;2. Networking Research Center on Bioengineering, Biomaterial and Biomedicine (CIBER-BBN), Spain;3. Department of Applied Physics, AM-UEx Research Group, Faculty of Science-UEX, Badajoz, Spain |
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| Abstract: | A fluorescent microbead-based immunoassay (FMIA) for simultaneous detection of IgG antibodies against Torque teno sus virus 1 (TTSuV1), TTSuV2, porcine reproductive and respiratory syndrome virus type 1 (PRRSV-1) and PRRSV-2 was developed. Serum samples were obtained over time from 20 pigs. Twelve of 20 were exposed to TTSuV2 on day 0, 20/20 were vaccinated with a PRRSV-2 vaccine on day 35, and 20/20 were exposed to PRRSV-2 on day 63. Anti-TTSuV antibodies were detected in 30% of the pigs on day 0, and 90% by day 35. All PRRSV-2 vaccinated pigs had detectable anti-PRRSV-2 IgG 21 days after vaccination. Field samples from 17 farms were also tested. The seroprevalence of both PRRSV and TTSuV increased with age. Comparison of the PRRSV-2 FMIA to an ELISA revealed good correlation in young pigs but a high rate of false positives in older pigs. Cross-reaction between PRRSV types was a problem. |
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