| 抗生素对高粱离体培养反应的影响 |
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| 引用本文： | 张明洲,崔海瑞,舒庆尧,夏英武.抗生素对高粱离体培养反应的影响[J].核农学报,2004,18(2):113-117. |
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| 作者姓名： | 张明洲 崔海瑞 舒庆尧 夏英武 |
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| 作者单位： | 浙江大学原子核农业科学研究所,浙江,杭州,310029;浙江大学原子核农业科学研究所,浙江,杭州,310029;浙江大学原子核农业科学研究所,浙江,杭州,310029;浙江大学原子核农业科学研究所,浙江,杭州,310029 |
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| 基金项目： | 安徽省合肥市政府科研专项 |
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| 摘 要： | 探讨了 5种抗生素对高粱离体培养反应的影响。在 3种抑菌抗生素中 ,羧苄青霉素和头孢霉素的抑菌效果较好。头孢霉素对高梁外植体离体培养的毒性比羧苄青霉素大 ,羧苄青霉素对愈伤组织的生长起促进作用 ,且只在高浓度才对分化有抑制作用。在农杆菌介导高梁遗传转化时 ,选用 2 5 0～ 5 0 0mg L的羧苄青霉素来抑菌是合适的。高梁不同基因型和外植体对潮霉素和卡那霉素的反应表现不同 ,但对潮霉素的反应比卡那霉素敏感。在筛选转化体时 ,以潮霉素较好 ,使用浓度对茎尖和幼胚以 2 5～ 5 0mg L为宜 ,对愈伤组织则以 5 0～ 75mg L为宜。
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| 关 键 词： | 高梁 离体培养 抗生素 |
| 文章编号： | 1000-8551(2004)02-113-05 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2003年3月25日 |
EFFECTS OF ANTIBIOTICS ON THE RESPONSE OFSORGHUM IN VITRO CULTURE |
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| ZHANG Ming-zhou CUI Hai-rui SHU Qing-yao XIA Ying-wu.EFFECTS OF ANTIBIOTICS ON THE RESPONSE OFSORGHUM IN VITRO CULTURE[J].Acta Agriculturae Nucleatae Sinica,2004,18(2):113-117. |
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| Authors: | ZHANG Ming-zhou CUI Hai-rui SHU Qing-yao XIA Ying-wu |
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| Abstract: | Effects of five kinds of antibiotics on the response of sorghum in vitro culture were studied.Among three kinds of antibiotics,carbenicillin and cefotaxime showed better results than ampicillin for the removal of Agrobacterium cells maintained in explants after co-culture.Higher toxicity of cefotaxime to sorghum explants was observed than that of carbenicillin,which could promote callus growth and only showed inhibition to plantlet regeneration under higher concentration.Thus,250～500mg/L of carbenicillin is optional for the removal of bacterial cells in sorghum transformation mediated by Agrobacterium.Sensitivity to kanamycin and hygromycin varied with the sorghum varieties and explants,but they are all more sensitive to hygromycin than to kanamycin.For screening transformast,hygromycin is preferable with the concentration of 25～50mg/L to shoot apex and immature embryo and 50～75mg/L to callus. |
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| Keywords: | sorghum in vitro culture antibiotics |
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