| 用AFLP技术构建棉花雄性不育三系及其杂种F1的DNA指纹图谱 |
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| 引用本文： | 朱云国,王学德,李悦有.用AFLP技术构建棉花雄性不育三系及其杂种F1的DNA指纹图谱[J].棉花学报,2001,13(3):158-160. |
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| 作者姓名： | 朱云国 王学德 李悦有 |
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| 作者单位： | 浙江大学农学系,杭州,310029 |
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| 基金项目： | 转基因植物研究及产业化(J00-B-002-10) |
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| 摘 要： | 实验应用AFLP同位素标记法,获得了棉花细胞质雄性不育系、保持系、恢复系及其杂种F1的DNA指纹图谱.较为详尽地介绍了有关AFLP的技术核心及注意点,并探讨了其适用于棉花研究的方法.结果表明,AFLP是一种十分有效的DNA指纹技术,它具有多态性丰富、稳定性高、重复性好等优点.
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| 关 键 词： | 棉花 细胞质 雄性不育 DNA图谱 AFLP |
| 文章编号： | 1002-7807(2001)03-0158-03 |
| 修稿时间： | 2000年10月25 |
DNA Fingerprinting Analysis of Cytoplasmic Male Sterile, Maintainer, Restorer Line and Hybrid (F1) in Upland Cotton by Using AFLP Technique |
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| ZHU Yun guo,WANG Xue de,LI Yue you.DNA Fingerprinting Analysis of Cytoplasmic Male Sterile, Maintainer, Restorer Line and Hybrid (F1) in Upland Cotton by Using AFLP Technique[J].Cotton Science,2001,13(3):158-160. |
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| Authors: | ZHU Yun guo WANG Xue de LI Yue you |
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| Abstract: | DNA fingerprinting of male sterile, maintainer, restorer line and hybrid in upland cotton was made by AFLP analysis. A protocol of AFLP analysis for detection of PCR amplified products was also described in this paper. The results of experiment showed that AFLP is an efficient technique for biodiversity analysis and fingerprinting. |
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| Keywords: | upland cotton cytoplasmic male sterility DNA fingerprinting AFLP |
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