| 大花萱草新品种“御衣黄”组织培养及植株再生 |
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| 引用本文： | 何月秋,祝志勇.大花萱草新品种“御衣黄”组织培养及植株再生[J].江苏林业科技,2011,38(3):12-16. |
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| 作者姓名： | 何月秋 祝志勇 |
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| 作者单位： | 宁波城市职业技术学院环境学院,浙江宁波,315502 |
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| 基金项目： | 宁波市农业科研公关项目 |
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| 摘 要： | 以大花萱草“御衣黄”花蕾为外植体建立其组织培养再生体系.研究结果表明,大花萱草“御衣黄”花蕾经75％乙醇30 s +0.1％升汞10 min处理后可获得较为理想的灭菌效果；诱导培养基MS+ 6-BA 5 mg/L+ NAA 5mg/L是花蕾理想的脱分化培养基,愈伤组织诱导率达到75％；在降低了激素质量浓度的培养基MS+...
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| 关 键 词： | 大花萱草“御衣黄” 花蕾 愈伤组织 植株再生 |
Tissue culture and plant regeneration of Hemerocallis middendorfii |
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| HE Yue-qiu,ZHU Zhi-yong.Tissue culture and plant regeneration of Hemerocallis middendorfii[J].Journal of Jiangsu Forestry Science & Technology,2011,38(3):12-16. |
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| Authors: | HE Yue-qiu ZHU Zhi-yong |
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| Institution: | (Faculty of Environmental Science,Ningbo City College of Vocational Technology,Ningbo 315502,China) |
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| Abstract: | An in vitro plant regeneration system of Hemerocallis middendorfii was established using buds as explants in the article.Results showed that the optimum sterilization method was 75% alcohol soaking 30 seconds and 0.1% HgCl2 treatment 10 minutes for explants.The optimum callus inducing medium was Murashige and Skoog（MS） ＋ NAA 0.5 mg/L ＋ 6-BA 3.0 mg/L with an induction rate of 75%.Callus was proliferated and adventitious buds were effectively generated on MS ＋ 6-BA 1.5 mg/L ＋ NAA 0.3 mg/L with a differentiation rate of 45.7%.The best rooting medium was 1/2 MS ＋ NAA 0.1 mg/L with a rooting rate 91.7%.The plantlet was transplanted with a transplanting survival rates up to 100% after one month. |
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| Keywords: | Hemerocallis middendorfii Buds Callus Plant regeneration |
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