Superovulatory response and embryo quality in Katahdin ewes treated with FSH or FSH plus eCG during non-breeding season |
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Authors: | Luna-Palomera C Macías-Cruz U Sánchez-Dávila F |
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Institution: | 1.Laboratory of Animal Reproduction and Genetic, Academic Division of Animal Sciences, Universidad Juárez Autónoma de Tabasco, Villahermosa, Tabasco, México ;2.Instituto de Ciencias Agrícolas, Universidad Autónoma de Baja California, Mexicali, México ;3.Laboratory of Animal Reproduction, Faculty of Agricultural Science, Universidad Autónoma de Nuevo León, Marín, N.L, México ; |
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Abstract: | The aim of this study was to evaluate the effect of a co-treatment of follicle-stimulating hormone (FSH) plus equine chorionic gonadotrophin (eCG) on serum insulin and insulin-like growth factor 1 (IGF-1) concentrations, superovulatory response, ovulatory rate, and number and production of embryos in Katahdin breed ewes during the non-breeding season. Twenty Katahdin ewes were synchronized with progestagens (CIDR) and assigned to two superovulation treatments (n?=?10): (1): ewes treated with 200 mg ewe?1 of FSH from day 5 to 8 after CIDR insertion at decreasing doses every 12 h (FSH group) and (2) ewes treated as FSH group plus 300 IU of eCG on day 5 after CIDR insertion (FSH + eCG group). Estrous behavior was monitored and direct mating was performed. On days ??7 (CIDR insertion), 0 (CIDR withdrawal), and 7 (embryo recovery), blood samples were collected to determine serum hormone concentrations. Co-treatment with eCG (FSH group) did not affect (P >?0.05) serum hormone levels. Superovulation response, ovulation rate, recovery rate, fertilization, and number of embryos were also similar (P >?0.05) between treatments. Compared with FSH group, FSH + eCG ewes had lower (P?<?0.05) number of transferable embryos and higher (P?<?0.05) number of oocyte and a tendency to increase the number of degenerated embryos (P?=?0.07). Overall results suggest that the administration of eCG is not beneficial either to improve the ovulatory response or the amount of transferable embryos in Katahdin ewes superovulated with a protocol using progesterone and FSH at decreasing doses. |
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