| 不同培养基的酵母发酵液对细菌纤维素产量的影响 |
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| 引用本文： | 何晓磊,刘四新,邓健,杨一冲,毕继才,刘龙祥,郝婧,李从发.不同培养基的酵母发酵液对细菌纤维素产量的影响[J].热带作物学报,2016,37(3):488-492. |
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| 作者姓名： | 何晓磊 刘四新 邓健 杨一冲 毕继才 刘龙祥 郝婧 李从发 |
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| 作者单位： | 1. 海南大学食品学院,海南海口 570228;海南大学环境与植物保护学院,海南海口 570228;2. 海南大学食品学院,海南海口 570228;海南大学材料与化工学院,海南海口 570228;3. 海南大学食品学院,海南海口,570228 |
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| 基金项目： | 海南省自然科学基金项目（No. 314048）；海南省普通高校研究生创新科研课题（No. Hy2013-B02）；海口市热带农产品深加工重点实验室项目（No. 2013-45）；海南大学研究生优秀学位论文培育计划。 |
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| 摘 要： | 为了提高细菌纤维素(BC)产量,研究4种酵母菌在NCW、PDB、YPD和BS等4种不同培养基中的发酵液对Gluconacetobacter europaeus Y9 BC产量的影响,并与新鲜椰子水和自然发酵椰子水发酵生产BC的产量进行对比。结果表明:在YPD培养基制成的4种酵母菌发酵液中BC产量都是最高的,其中活性干酵母贝酵母、面包酵母和自行分离筛选的野生酵母威克克鲁维酵母、热带假丝酵母生产的BC产量分别为6.91、5.53、6.15、4.67 g/L,都显著高于以新鲜椰子水发酵合成的BC产量,贝酵母和威克克鲁维酵母在不同培养基中对BC产量影响不同,可能高于或者是接近自然发酵椰子水中的BC产量。推测酵母菌发酵液促进BC合成的原因不仅由于产生了合适浓度的乙醇,应该还与酵母菌产生了其他代谢产物,并且提供了良好的缓冲能力等有关。另一方面,筛自自然发酵椰子水的、野生的威克克鲁维酵母在任何培养基中培养后BC产量都较高,推测该菌株可能是新鲜椰子水经自然发酵后能使BC产量大幅度提高的重要贡献菌种之一。
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| 关 键 词： | 细菌纤维素 椰纤果 酵母发酵液 产量增加 椰子水 |
Effects of Yeast Fermentation in Different Kinds of Media on Production of Bacterial Cellulose
by Gluconacetobacter europaeus Y9 |
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| HE Xiaolei,LIU Sixin,DENG Jian,YANG Yichong,BI Jicai,LIU Longxiang,HAO Jing and LI Congfa.Effects of Yeast Fermentation in Different Kinds of Media on Production of Bacterial Cellulose
by Gluconacetobacter europaeus Y9[J].Chinese Journal of Tropical Crops,2016,37(3):488-492. |
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| Authors: | HE Xiaolei LIU Sixin DENG Jian YANG Yichong BI Jicai LIU Longxiang HAO Jing and LI Congfa |
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| Institution: | 1 College of Food Science and Technology, Hainan University 2 College of Environment and Plant Protection, Hainan University,;1 College of Food Science and Technology, Hainan University;College of Materials and Chemical Engineering, Hainan University;College of Materials and Chemical Engineering, Hainan University;College of Materials and Chemical Engineering, Hainan University;College of Materials and Chemical Engineering, Hainan University;College of Materials and Chemical Engineering, Hainan University;College of Materials and Chemical Engineering, Hainan University |
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| Abstract: | To improve the yield of BC, the study compared the BC yield by Gluconacetobacter europaeus Y9 from four different media(NCW, PDB, BS and YPD)with four different strains of yeast, and it was also compared with NCW and FCW. The results showed that: BC yield was the highest from YPD for all yeast fermented broth, and BC yield of YPD treated with active dry yeast Saccharomyces bayanus, Saccharomyces cerevisiae, self-isollated wild-type Kluyveromyces wickerhamii and Candida tropicalis was 6.91, 5.53, 6.15, 4.67 g/L respectively, all significantly higher than that from NCW. BC yield from S. bayanus and K. wickerhamii fermented broth were higher than FCW, indicating that the improved BC yield from fermented broth was not only suitable concentration of ethanol produced, but also some other metabolites and the suitable buffered condition. On the other hand, the BC yield from medium treated with K. wickerhamii was relatively high for all culture medium, suggesting that this yeast strain might be one of the key contributor to the improved BC yield in naturally fermented coconut water. |
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| Keywords: | Bacterial cellulose Nata de coco Yeast fermentation Enhanced production Coconut water |
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