| 海南龙血树类黄酮3′-羟化酶基因(DcF3′H)的克隆与表达分析 |
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| 引用本文： | 陈盼,曹天骏,戴好富,李辉亮,郭冬,梅文莉,彭世清.海南龙血树类黄酮3′-羟化酶基因(DcF3′H)的克隆与表达分析[J].热带作物学报,2016,37(3):568-575. |
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| 作者姓名： | 陈盼 曹天骏 戴好富 李辉亮 郭冬 梅文莉 彭世清 |
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| 作者单位： | 1. 海南大学农学院,海南海口 570228;中国热带农业科学院热带生物技术研究所 农业部热带作物生物学与遗传资源利用重点实验室 海南海口 571101;2. 中国热带农业科学院热带生物技术研究所 农业部热带作物生物学与遗传资源利用重点实验室 海南海口 571101 |
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| 基金项目： | 公益性行业（农业）科研专项（No. 201303117）；国家科技支撑计划课题(No. 2013BAI11B04)；海南省重大科技项目(No. ZDZX2013008-4)。 |
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| 摘 要： | 类黄酮3′-羟化酶(Flavonoid 3′-hydroxylase,F3′H)是植物黄酮类化合物骨架修饰途径中的关键酶之一,在形成结构多样化的黄酮类化合物中起重要作用。在前期已获得的龙血树转录组数据基础上克隆了1个海南龙血树F3′H基因,命名为DcF3′H。DcF3′H含有的开放阅读框长1 533 bp,编码510个氨基酸。推导的DcF3′H分子量为58 ku,等电点p I为6.59。DcF3′H含有细胞色素P450的保守结构域和CYP基序,与甘蔗、玉米、猕猴桃、小麦、烟草、黄瓜和大豆等植物的F3′H同源性分别为76%、75%、77%、74%、75%、76%和75%。进化树分析表明,DcF3′H与中国水仙亲缘关系较近,与苜蓿和鹰嘴豆的亲缘关系较远。荧光定量表达结果显示:DcF3′H在根、茎、叶、花和果等组织中均有表达,其中在花中表达量最高,根中表达量最低;无机盐诱导剂处理能显著提高DcF3'H的表达量,处理3 d时DcF3′H的表达量提高了5.58倍。此结果将为进一步研究海南龙血树类黄酮3′-羟化酶基因的功能奠定基础。
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| 关 键 词： | 海南龙血树 类黄酮3'-羟化酶 表达分析 |
Cloning and Expression Analysis of Flavonoid 3'-Hydroxylase (F3'H) Gene in Dracaena cambodiana Pierre ex Gagnep |
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| CHEN Pan,CAO Tianjun,DAI Haofu,LI Huiliang,GUO Dong,MEI Wenli and PENG Shiqing.Cloning and Expression Analysis of Flavonoid 3'-Hydroxylase (F3'H) Gene in Dracaena cambodiana Pierre ex Gagnep[J].Chinese Journal of Tropical Crops,2016,37(3):568-575. |
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| Authors: | CHEN Pan CAO Tianjun DAI Haofu LI Huiliang GUO Dong MEI Wenli and PENG Shiqing |
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| Institution: | 1 College of Agriculture, Hainan University 2 Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;1 College of Agriculture, Hainan University 2 Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture/Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences |
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| Abstract: | |
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| Keywords: | Dracaena cambodiana Flavonoid 3'-hydroxylase Expression analysis |
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