| 香蕉MaTPS4基因序列及表达特性分析 |
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| 引用本文： | 许桂莺,徐碧玉,邢文婷,孙威,王安邦,宋顺,陈青,李科明.香蕉MaTPS4基因序列及表达特性分析[J].热带作物学报,2016,37(3):532-538. |
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| 作者姓名： | 许桂莺 徐碧玉 邢文婷 孙威 王安邦 宋顺 陈青 李科明 |
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| 作者单位： | 1. 中国热带农业科学院海口实验站,海南海口 570102;中国热带农业科学院橡胶研究所,海南儋州 571737;华中农业大学,湖北武汉 430070;2. 中国热带农业科学院热带作物生物技术研究所,海南海口,571101;3. 海南省林业科学研究所,海南海口,571100;4. 中国热带农业科学院海口实验站,海南海口,570102;5. 中国热带农业科学院橡胶研究所,海南儋州,571737 |
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| 基金项目： | 农业部重点实验室开放式课题（No. RRI-KLOF1403）；“十二五”农村领域国家科技计划（No. 2011AA10020605）；现代农业产业技术体系建设专项（No. CARS-32）；海南省耕地改良关键技术研究与示范专项（No. HNGDpz2015）。 |
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| 摘 要： | 通过随机克隆测序法从香蕉根系c DNA文库中获得海藻糖合成酶基因,命名为MaTPS4,全长c DNA序列为2 930 bp,含一个完整的开放阅读框2 574 bp,编码857个氨基酸。生物学信息分析表明,MaTPS4蛋白属于不稳定、疏水性蛋白,pI5.59,结构域TPS和TPP。序列预测分析表明,MaTPS4蛋白定位于细胞质中,具有3个跨膜区域,不存在信号肽。与其他已知植物TPS氨基酸序列同源比对,同源性达到84.90%;进化关系分析表明,香蕉MaTPS4氨基酸与银杏TPS氨基酸序列(AAX16014.1)聚为一类,二者亲缘关系较近,同源性为99%。器官特异性分析表明,MaTPS4在香蕉根系、球茎、假茎、叶、花、果实各器官中均有表达,其中球茎、假茎、叶片和花中表达量较大,根及果实中表达量极少。q RT-PCR分析表明,MaTPS4在根系中的表达经干旱、盐胁迫后均增强,其中盐胁迫24 h时MaTPS4表达量较0 h时高5倍;冷胁迫下,MaTPS4表达量增加,并随时间延长下降,在6 h达到最大;ABA胁迫下,MaTPS4表达量增加,为0 h的5倍;在ACC和Foc TR4胁迫下,MaTPS4表达无变化。因此,MaTPS4可能通过依赖ABA途径调控抗逆胁迫相关基因表达,提高植株对非生物胁迫的抗逆性。
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| 关 键 词： | 香蕉 海藻糖-6-磷酸合成酶基因(TPS4) 生物信息学 表达分析 |
Sequence and Expression Characteristics Analysis of Banana MaTPS4 |
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| XU Guiying,XU Biyu,XING Wenting,SUN Wei,WANG Anbang,SONG Shun,CHEN Qing and LI Keming.Sequence and Expression Characteristics Analysis of Banana MaTPS4[J].Chinese Journal of Tropical Crops,2016,37(3):532-538. |
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| Authors: | XU Guiying XU Biyu XING Wenting SUN Wei WANG Anbang SONG Shun CHEN Qing and LI Keming |
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| Institution: | 1 Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences 2 Institute of Rubber Research, Chinese Academy of Tropical Agricultural Sciences 4 Huazhong Agricultural University;Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences;Hainan Pprovincial Forestry Science Institute;Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences;Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences;Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences;Institute of Rubber Research, Chinese Academy of Tropical Agricultural Sciences;Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences |
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| Abstract: | A full-length 2 930 bp of trehalose -6- phosphate synthetase gene 4 was obtained from the cDNA of banana roots, which was named as MaTPS4. It contained a length 2 574 bp of open reading frame which encoding 857amino acids. Biological information analysis suggested that MaTPS4 protein was an unstable protein with isoelectric point of 5.59 and two domains TPS and TPP. Sequence forecasting analysis indicated MaTPS4 localized in cytoplasm and had three trans-membrane regions without signal peptides. The amino acid sequence that MaTPS4 encoded had homology level of 83.80% with the known TPS amino acid sequence of other plants. Phylogenetic analysis indicated MaTPS4 was closer with Ginkgo biloba(AAX16014.1), and the homology level was 99%. Organ-specific analysis showed MaTPS4 were expressed in banana roots, corms, pseudo-stems, leaves, flowers and fruits, and higher in corms, pseudo-stems and flowers, but lower in roots and fruits. qRT-PCR analysis showed the expression level of MaTPS4 increased under drought and salt stress, and was 5 times higher than 0 h, when banana planets were treated at 24 h; and decreased when was treated under cold stress; and increased after ABA treated the planets, the expression level of MaTPS4 was 5 times than 0 h. The expression of MaTPS4 was not variation under ACC and FocTR4 treatment. Therefore, MaTPS4 may be involved in banana plant defense responses through participation in the regulation of ABA, and ABA-dependent stress related genes to improve plant resistance. |
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| Keywords: | Banana Trehalose-6-phosphate synthase gene(TPS4) Bioinformatics: Expression analysis |
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