Chondroitin AC lyase activity is related to virulence of fish pathogenic Flavobacterium columnare |
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| Authors: | Suomalainen L-R Tiirola M Valtonen E T |
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| Institution: | Department of Biological and Environmental Science, University of Jyv?skyl?, Jyv?skyl?, Finland. lottari@bytl.jyu.fi |
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| Abstract: | The virulence of eight Flavobacterium columnare strains was studied to find correlations between several virulence-related factors and virulence. Virulence was tested in vivo using rainbow trout, Oncorhynchus mykiss (Walbaum). Suggested virulence-related factors such as production of the degradative enzyme chondroitin lyase, plasmid occurrence and adhesion capability were studied in vitro. Infection with the four most virulent strains resulted in 95-100% mortality within 114 h. Chondroitin lyase activity was found to be significantly related to the virulence of the strains at 25 degrees C and it was also shown to be temperature-dependent, being higher at 25 degrees C than at 20 degrees C. Virulence was not plasmid associated. The adhesion capability of the strains in vitro varied substantially when tested on crude mucus-coated slides and no statistical relationship between adhesion and virulence was found using this method. |
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| Keywords: | adhesion chondroitin AC lyase Flavobacterium columnare plasmid rainbow trout virulence |
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