| 不同剂型苯甲酸在体外和体内的控释效果研究 |
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| 引用本文： | 不同剂型苯甲酸在体外和体内的控释效果研究.不同剂型苯甲酸在体外和体内的控释效果研究[J].畜牧与饲料科学,2022,43(6):42-47. |
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| 作者姓名： | 不同剂型苯甲酸在体外和体内的控释效果研究 |
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| 作者单位： | 广东酸动力生物科技有限公司，广东 清远 511500 |
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| 摘 要： | 目的]研究不同剂型苯甲酸的体外和体内控释效果。方法]试验分为体外溶出试验和体内控释试验。①体外溶出试验：配制人工胃液和人工肠液，在200目转篮中置入普通型苯甲酸和控释型苯甲酸，在溶出度仪中于1、2 h（人工胃液）和1、2、3、4 h（人工肠液）取样，采用高效液相色谱（HPLC）技术检测苯甲酸含量，计算溶出度和留存率。②体内控释试验：选用体重相近的健康清远麻肉鸡36只，预饲后分为3组，每组12只；晨饲后立即投喂纯面粉丸剂（对照组）、含0.1%普通型苯甲酸的面粉丸剂和含0.1%控释型苯甲酸的面粉丸剂，并于投喂后0、1、2、4 h每组分别取3只鸡屠宰，取各区段胃肠道内容物，采用HPLC技术检测苯甲酸含量。结果]①体外溶出试验：在人工胃液中1 h，控释型苯甲酸的留存率是普通型苯甲酸的3.45倍；在人工胃液中2 h，普通型苯甲酸未被检测出，而控释型苯甲酸的留存率为56.18%；在人工肠液中4 h，控释型苯甲酸的留存率为40.10%，而普通型苯甲酸在1 h时未被检测出。②体内控释试验：采食后1 h，控释型苯甲酸组在腺胃+肌胃中的苯甲酸浓度是普通型苯甲酸组的5.46倍，二者差异显著（P<0.05）；采食后2 h，控释型苯甲酸组在空肠、回肠、盲肠内容物中的苯甲酸浓度分别是普通型苯甲酸组的1.61、2.43、1.95倍，差异均达到显著（P<0.05）水平；采食后4 h，控释型苯甲酸组在回肠内容物中的苯甲酸浓度是普通型苯甲酸组的6.68倍，二者差异显著（P<0.05）。结论]体外溶出和体内控释试验均表明，将苯甲酸进行包被等工艺处理后形成的控释型苯甲酸具有较高的过胃率，进入肠道后段在整个肠道缓慢释放，实现长效抑菌功效。
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| 关 键 词： | 苯甲酸 溶出度 控释 人工模拟 |
| 收稿时间： | 2022-10-28 |
Controlled-releasing Performance of Benzoic Acid in Different Forms in Vitro and in Vivo |
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| Xiao-nan ZHAO,You-jun HU,Huang-zuo CHENG,Jian LIU,Xin-fang LU,Hai-yun LI.Controlled-releasing Performance of Benzoic Acid in Different Forms in Vitro and in Vivo[J].Animal Husbandry and Feed Science,2022,43(6):42-47. |
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| Authors: | Xiao-nan ZHAO You-jun HU Huang-zuo CHENG Jian LIU Xin-fang LU Hai-yun LI |
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| Institution: | Guangdong Nuacid Biotechnology Co.，Ltd.，Qingyuan 511500，China |
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| Abstract: | Objective] The present study aimed to evaluate the controlled-releasing performance of benzoic acid in different forms in vitro and in vivo. Method] This study consisted of two parts, an in vitro dissolution test and an in vivo controlled-release test. ① In vitro dissolution test: Artificial gastric liquid and artificial intestinal liquid was prepared, with ordinary benzoic acid or controlled-release benzoic acid in the 200 mesh baskets. Samples were taken from the dissolution meter at 1, 2 h for artificial gastric liquid or 1, 2, 3 and 4 h for artificial intestinal liquid. High-performance liquid chromatography （HPLC） was used to determine the content of benzoic acid, and the dissolution rate and retention rate were calculated. ② In vivo controlled-release test: A total of 36 healthy Qingyuanma broilers with similar weights were selected and assigned into 3 groups after pre-feeding, with 12 individuals in each group. After morning feeding, the broilers in control group, ordinary benzoic acid group and controlled-release benzoic acid group were immediately fed with pure flour pills, flour pills containing 0.1% ordinary benzoic acid and flour pills containing 0.1% controlled-release benzoic acid, respectively. On 0, 1, 2 and 4 h after given the pills, three broilers were chosen from each group, respectively. The broilers were slaughtered and the contents in each segment of gastrointestinal tracts were taken. The concentration of benzoic acid in the gastrointestinal contents of broilers were assessed by HPLC. Result]① In vitro dissolution test: In artificial gastric liquid for 1 h, the retention rate of controlled-release benzoic acid was 3.45 times that of ordinary benzoic acid. In artificial gastric liquid for 2 h, ordinary benzoic acid was not detected, while the retention rate of controlled-release benzoic acid was 56.18%. The retention rate of controlled-release benzoic acid was 40.10% in the artificial intestinal fluid for 4 h, while ordinary benzoic acid was not detected at 1 h. ② In vivo controlled release test: The concentration of benzoic acid in glandular stomach and muscular stomach of the controlled-release benzoic acid group was 5.46 times of the ordinary benzoic acid group 1 h post ingestion of the pills, exhibiting a significant （P<0.05） difference. The concentration of benzoic acid in contents of jejunum, ileum and cecum of the controlled-release benzoic acid group 2 h post ingestion of the pills was 1.61, 2.43 and 1.95 times of the ordinary benzoic acid group, respectively, all exhibiting significant （P<0.05） differences. The concentration of benzoic acid in ileum contents of the controlled-release benzoic acid group was 6.68 times of the ordinary benzoic acid group 4 h post ingestion of the pills, exhibiting a significant （P<0.05） difference. Conclusion] Both in vitro dissolution test and in vivo controlled-release test showed that the controlled-release benzoic acid prepared by coating treatment had a high gastric pass rate and was able to slowly release into the entire intestinal tract after entering to achieve long-term antibacterial effect. |
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| Keywords: | benzoic acid dissolution rate controlled-release artificial simulation |
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