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| 萝卜ALMT基因家族的鉴定与表达 | |
| 引用本文： | 刘同金,徐铭婕,汪精磊,刘良峰,崔群香,包崇来,王长义.萝卜ALMT基因家族的鉴定与表达[J].浙江农业学报,2022,34(4):746-755. |
| 作者姓名： | 刘同金 徐铭婕 汪精磊 刘良峰 崔群香 包崇来 王长义 |
| 作者单位： | 1.金陵科技学院 园艺园林学院,江苏 南京 2100382.浙江省农业科学院 蔬菜研究所,浙江 杭州 3100213.江宁区农业农村局,江苏 南京 211100 |
| 基金项目： | 国家自然科学基金(31801858);国家级大学生创新训练计划(202113573014Z);金陵科技学院高层次人才科研启动项目(jit-b-202009);国家现代农业产业技术体系(CARS-23-A-14) |
| 摘 要： | 为探究萝卜铝激活苹果酸转运蛋白(ALMT)家族成员对生物和非生物胁迫的响应,本研究通过生物信息学方法对萝卜ALMT家族成员进行鉴定,并利用转录组数据对其进行表达分析.结果显示,萝卜基因组中包含17个ALMT基因,分布于8条染色体.该家族成员外显子数量为5～7个,预测N-端含有5～6个跨膜结构,在染色体上的分布不均匀.萝... |
| 关 键 词： | 萝卜 ALMT基因家族 系统进化 基因表达 |
| 收稿时间： | 2021-07-30 |
Genome-wide identification and expression analysis of ALMT gene family in radish |
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| LIU Tongjin,XU Mingjie,WANG Jinglei,LIU Liangfeng,CUI Qunxiang,BAO Chonglai,WANG Changyi.Genome-wide identification and expression analysis of ALMT gene family in radish[J].Acta Agriculturae Zhejiangensis,2022,34(4):746-755. | |
| Authors: | LIU Tongjin XU Mingjie WANG Jinglei LIU Liangfeng CUI Qunxiang BAO Chonglai WANG Changyi |
| Institution: | 1. College of Horticulture, Jinling Institute of Technology, Nanjing 210038, China 2. Institute of Vegetable, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China 3. Agricultural and Rural Affairs Bureau of Jiangning District, Nanjing 211100, China |
| Abstract: | In order to explore the response of aluminium activated malate transporter (ALMT) genes to biotic and abiotic stress in radish,ALMT genes of radish were genome-wide identified by bioinformatics method and expression patterns were analyzed based on RNA-seq data. The results showed that there were 17 ALMT genes in radish genome, and distributed on eight chromosomes. Exon number of these genes ranged from 5 to 7, and 5-6 transmembrane domains were predicted in their N-terminal region. Number of RsALMT genes on chromosomes was different. Expression analysis of RsALMT genes in different tissues and developments stages,treatment with Agrobacterium tumefaciens and heavy metal (cadmium, chromium and lead) showed that RsALMT1 and RsALMT13 might be involved in resistance to the infection of A. tumefaciens, RsALMT3 might control stomatal open, while RsALMT7, RsALMT11 and RsALMT14 mightbe involved in stomatal closure.RsALMT15 might regulate the accumulation of chlorophyll in flesh of radish and RsALMT16 might be involved in resistance to leadstress.Therefore, the above results would provide a theoretical basis for further study on the functions and molecular mechanisms of radish ALMT family members in response to biotic and abiotic stress. |
| Keywords: | radish ALMT gene family phylogenetic evolution gene expression |
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