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| CRISPR/Cas9基因编辑技术在植物中的应用与政策监管 | |
| 引用本文： | 偶春,张敏,丁霖,姚侠妹,王泽璐,彭城,徐俊锋.CRISPR/Cas9基因编辑技术在植物中的应用与政策监管[J].浙江农业学报,2022,34(8):1806. |
| 作者姓名： | 偶春 张敏 丁霖 姚侠妹 王泽璐 彭城 徐俊锋 |
| 作者单位： | 1.阜阳师范大学 生物与食品工程学院,安徽 阜阳 2360372.浙江省农业科学院 浙江省农产品质量安全危害因子与风险防控国家重点实验室,浙江 杭州 3100213.安徽建筑大学 建筑与规划学院,安徽 合肥 230022 |
| 基金项目： | 浙江省基础公益研究计划(LGN22C130015);浙江省农业新品种选育重大科技专项(2021C02064-6);安徽省高校自然科学研究重点项目(KJ2020A0519);安徽省高校自然科学研究重点项目(KJ2020A0456) |
| 摘 要： | 基因编辑技术是一种在基因组水平上对DNA序列进行精准修饰,从而促使基因组序列定向改造的技术。随着近几年CRISPR/Cas9技术的快速发展,基因组编辑技术在作物育种领域发挥了越来越重要的作用。本文综述了基因编辑技术的发展历程,以及CRISPR/Cas9的工作原理,分析了CRISPR/Cas9的局限性并提出了改进方法。重点阐述了植物CRISPR/Cas9基因编辑体系的建立、在植物性状改良方面的应用,以及最终致力于基因编辑产品商业化的应用案例。同时还分析了美国、欧盟、英国、日本和中国这5个代表性国家/地区的基因编辑监管政策和态度,以期为我国科学监管框架的建立提供参考,促进CRISPR/Cas9在我国乃至全球的产业化应用。 |
| 关 键 词： | 基因编辑技术 CRISPR/Cas9 性状改良 局限性 监管政策 |
| 收稿时间： | 2021-11-03 |
Application and policy regulation of CRISPR/Cas9 gene editing technology in plants |
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| OU Chun,ZHANG Min,DING Lin,YAO Xiamei,WANG Zelu,PENG Cheng,XU Junfeng.Application and policy regulation of CRISPR/Cas9 gene editing technology in plants[J].Acta Agriculturae Zhejiangensis,2022,34(8):1806. | |
| Authors: | OU Chun ZHANG Min DING Lin YAO Xiamei WANG Zelu PENG Cheng XU Junfeng |
| Institution: | 1. College of Biological and Food Engineering, Fuyang Normal University, Fuyang 236037, Anhui, China 2. State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China 3. School of Architecture & Urban Planning, Anhui Jianzhu University, Hefei 230022, China |
| Abstract: | Gene editing technology is a technology that making precise modification of DNA sequence at the genome level, so as to promote targeted modification of the genome sequence. With the rapid development of CRISPR/Cas9 technology in recent years, gene editing technology plays an increasingly important role in crop breeding. The paper reviewed the development of gene editing technology and the working principle of CRISPR/Cas9, analysed the limitations of CRISPR/Cas9 and put forward improvement methods. In addition, this paper focused on the establishment of the plant CRISPR/Cas9 gene editing system, its application in plant trait improvement, and the application cases that were finally committed to the commercialization of gene editing products. At the same time, it also analysed the regulatory policies and attitudes of gene editing in five representative countries/regions, including the United States, the European Union, Britain, Japan and China, in order to provide reference for the establishment of scientific regulatory frame work in China and promote the industrial application of CRISPR/Cas9 in China or even the world. |
| Keywords: | gene editing technology CRISPR/Cas9 traits improvement limitation regulatory policy future prospects |
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