| 简青霉CEF-818固体发酵工艺优化 |
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| 引用本文： | 蒲丹丹,李艳,赵丽红,张亚林,魏锋,冯鸿杰,朱荷琴,顾爱星,彭军,冯自力.简青霉CEF-818固体发酵工艺优化[J].中国生物防治学报,2022,38(3):636-644. |
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| 作者姓名： | 蒲丹丹 李艳 赵丽红 张亚林 魏锋 冯鸿杰 朱荷琴 顾爱星 彭军 冯自力 |
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| 作者单位： | 1. 新疆农业大学农学院/棉花教育部工程研究中心, 乌鲁木齐 830052;2. 中国农业科学院棉花研究所/棉花生物学国家重点实验室, 安阳 455000 |
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| 基金项目： | 国家棉花产业技术体系（CARS-15-22）；棉花生物学国家重点实验室自主课题（CB2021C12）；中国农业科学院科技创新工程（CAAS-ASTIPCCRI） |
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| 摘 要： | 简青霉Penicillium simplicissimum CEF-818发酵产物能够有效的防治由大丽轮枝菌Verticillium dahliae引起的棉花黄萎病，为获得简青霉CEF-818最佳的发酵条件，本研究采用单因素和正交试验方法对简青霉CEF-818固体发酵培养基的组成以及发酵条件进行了优化。结果表明，简青霉CEF-818固体发酵最适培养物为麦麸，其他添加物为葡萄糖、尿素和氯化钾，添加量按麦麸的质量比分别为2.5%、0.6%和0.6%（M/M），料水比为1：0.5，固体发酵最佳的培养条件为液体接种量为5%（V/M）、初始pH为7、物料厚度为2 cm、培养温度为28℃、培养时间为7 d。在此条件下，简青霉CEF-818的产孢量为8.28×109 CFU/g。
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| 关 键 词： | 生物防治 简青霉 固体发酵 产孢量 |
| 收稿时间： | 2021-12-21 |
Optimization of Solid-State Fermentation Process of Penicillium simplicissimum CEF-818 |
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| PU Dandan,LI Yan,ZHAO Lihong,ZHANG Yalin,WEI Feng,FENG Hongjie,ZHU Heqin,GU Aixing,PENG Jun,FENG Zili.Optimization of Solid-State Fermentation Process of Penicillium simplicissimum CEF-818[J].Chinese Journal of Biological Control,2022,38(3):636-644. |
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| Authors: | PU Dandan LI Yan ZHAO Lihong ZHANG Yalin WEI Feng FENG Hongjie ZHU Heqin GU Aixing PENG Jun FENG Zili |
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| Institution: | 1. Engineering Research Centre of Cotton, Ministry of Education/College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China;2. State Key Laboratory of Cotton Biology/Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, China |
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| Abstract: | The fermentation product of Penicillium simplicissimum CEF-818 can effectively control cotton Verticillium wilt caused by Verticillium dahliae. In order to optimize fermentation conditions of P. simplicissimum CEF-818, the composition of solid fermentation medium and fermentation conditions of CEF-818 were screened by single factor and orthogonal test. The results showed that the optimum culture for solid fermentation of P. simplicissimum CEF-818 was wheat bran, and addition of 2.5% glucose (M/M), 0.6% urea (M/M) and 0.6% potassium chloride (M/M), with the initial pH value of 7.0, the ratio of material to water 1:0.5, the liquid inoculums level of 5% (V/M), the material thickness of 2 cm and at 28℃ for 7 days. Under this optimal medium and fermentation process, the sporulation could reach to 8.28×109 CFU/g for P. simplicissimum CEF-818. |
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| Keywords: | biocontrol Penicillium simplicissimum solid fermentation sporulation |
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