| 关防风立枯病病原菌根际拮抗细菌筛选与鉴定 |
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| 引用本文： | 陈逢玲,于航,常丽娜,孙卓,林红梅,杨利民.关防风立枯病病原菌根际拮抗细菌筛选与鉴定[J].中国生物防治学报,2022,38(3):681-688. |
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| 作者姓名： | 陈逢玲 于航 常丽娜 孙卓 林红梅 杨利民 |
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| 作者单位： | 吉林农业大学中药材学院/省部共建生态恢复与生态系统管理国家重点实验室, 长春 130118 |
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| 基金项目： | 国家重点研发计划项目（2019YFC1710700）；财政部和农业农村部国家现代农业产业技术体系资助（CARS-21）；吉林省科技发展计划项目（20200402104NC、20200404010YY、20210204011YY） |
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| 摘 要： | 为筛选出对立枯丝核菌Rhizoctonia solani Kühn具有优良防效的拮抗菌株。本研究从关防风根际土壤中分离纯化出156株细菌，采用平板对峙法测定菌株SC-32和SC-127对立枯丝核菌均有良好拮抗效果，且兼具广谱抑菌能力；抗生素标记法测定菌株SC-32和SC-127接种35 d后在土壤中含菌量仍能保持2.93×107和7.83×106CFU/g；通过盆栽试验研究结果表明，菌株SC-32和SC-127对关防风立枯病的防治效果为60.53%和65.89%，高于菌剂和农药处理。通过形态学、生理生化特征、16S rDNA基因序列进行分类学鉴定确定菌株SC-32和SC-127均为贝莱斯芽胞杆菌Bacillus velezensis。
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| 关 键 词： | 关防风立枯病 拮抗细菌 筛选 定殖 防病 |
| 收稿时间： | 2021-10-12 |
Screening and Identification of the Antagonistic Bacteria against Saposhnikovia divaricata Seedling Damping-off |
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| CHEN Fengling,YU Hang,CHANG Lina,SUN Zhuo,LIN Hongmei,YANG Limin.Screening and Identification of the Antagonistic Bacteria against Saposhnikovia divaricata Seedling Damping-off[J].Chinese Journal of Biological Control,2022,38(3):681-688. |
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| Authors: | CHEN Fengling YU Hang CHANG Lina SUN Zhuo LIN Hongmei YANG Limin |
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| Institution: | Breeding Base of State Key Laboratory of JLP-MOST for Ecological Restoraton and Ecosystem Management/College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | This study aims to discover antagonistic strains with good control effect against Rhizoctonia solani. 156 bacterial strains were identified and purified from Saposhnikovia divaricata rhizosphere soil, and both strains SC-32 and SC-127 exhibited good antagonistic effects against R. solani by plate antagonism technique, as well as broad-spectrum antibacterial capacity. 35 days post antibiotic labeling inoculation, the quantities of SC-32 and SC-127 remained 2.93×107 and 7.83×106CFU/g in soil, respectively. Pot experiment revealed that SC-32 and SC-127 had 60.53% and 65.89% control efficacies on S. divaricata seedling damping-off, respectively, which were greater than thiophanate-methyl, Trichoderma harzianum, and Bacillus subtilis. Based on analyses of morphological, physiological, and biochemical properties, as well as the 16S rDNA gene sequence, the strains SC-32 and SC-127 were identified as Bacillus velezensis. |
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| Keywords: | Saposhnikovia divaricata seedling damping-off antagonistic bacterium screening colonization control effect |
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