| 植物提取物对油茶炭疽菌的抑菌活性筛选 |
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| 引用本文： | 罗建梅,张兴怡,伍建榕,刘丽,洪英娣,张东华,闫晓慧.植物提取物对油茶炭疽菌的抑菌活性筛选[J].中国生物防治学报,2022,38(4):852-859. |
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| 作者姓名： | 罗建梅 张兴怡 伍建榕 刘丽 洪英娣 张东华 闫晓慧 |
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| 作者单位： | 1. 云南省高校森林灾害预警控制重点实验室/西南林业大学生物多样性保护学院, 昆明 650224;2. 西南地区生物多样性保育国家林业局重点实验室/西南林业大学林学院, 昆明 650224 |
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| 基金项目： | 国家重点研发计划(2019YFD1002000);国家自然科学基金(31860208,32160378);云南省教育厅科学研究基金(11070733) |
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| 摘 要： | 为开发新型植物源农药,探究油茶炭疽病的生物防治方法,以油茶炭疽病菌(Colletotrichum fructicola)为供试病原菌,采用生长速率法测定了49种植物的70种提取物对油茶炭疽病菌的抑制作用,筛选出活性较好的提取物,并通过浓度梯度试验测定其抑菌毒力。结果表明,在提取物浓度为1 mg/mL时,臭灵丹石油醚萃取物和益母草乙酸乙酯萃取物对油茶炭疽菌抑菌效果最好,抑制率分别为54.48%和50.75%,经毒力测定其有效中浓度(EC50)分别为0.7013和0.6246 mg/mL。本研究为进一步利用臭灵丹、益母草开发植物源抑菌剂提供了一定的理论依据。
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| 关 键 词： | 植物提取物 生长速率法 毒力 油茶炭疽菌 抑菌活性 |
| 收稿时间： | 2022-06-26 |
Antifungal Activity of Plant extracts against Colletotrichum fructicola,Pathogen of Camellia oleifera |
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| LUO Jianmei,ZHANG Xingyi,WU Jianrong,LIU Li,HONG Yingdi,ZHANG Donghua,YAN Xiaohui.Antifungal Activity of Plant extracts against Colletotrichum fructicola,Pathogen of Camellia oleifera[J].Chinese Journal of Biological Control,2022,38(4):852-859. |
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| Authors: | LUO Jianmei ZHANG Xingyi WU Jianrong LIU Li HONG Yingdi ZHANG Donghua YAN Xiaohui |
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| Institution: | 1. Key Laboratory of Forest Disaster Warning and Control in Universities of Yunnan Province/College of Biodiversity Conservation, Southwest Forestry University, Kunming 650224, China;2. Key Laboratory of Biodiversity Conservation in Southwest China, State Forestry Administration/College of Biodiversity Forestry, Southwest Forestry University, Kuming 650224, China |
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| Abstract: | In order to study the biological control method of Colletotrichum fructicola and develop new botanical pesticides,the inhibitory effects of 70 extracts from 49 plants on C.fruticola were determined by growth rate method.The extracts with significant inhibition activity were screened out,and their antifungal toxicity were determined by concentration gradient test.Petroleum ether extract of Laggera pterodonta and ethyl acetate extract of Leonurus artemisia showed potent antifungal activity against C.fruticola, with inhibitory rates of 54.48% and 50.75%,respectively,at the concentration of 1 mg/mL,and their corresponding medium effective concentrations (EC50) were 0.7013 and 0.6246 mg/mL,respectively.The above results provided a theoretical basis for further development of botanical fungicides by using L.pterodonta and L.artemisia. |
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| Keywords: | plant extract growth rate method toxcity Colletotrichum fructicola antifungal activity |
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