| 金丝桃素对小鼠生长性能、脏器指数及盲肠微生物多样性的影响 |
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| 引用本文： | 金丝桃素对小鼠生长性能、脏器指数及盲肠微生物多样性的影响.金丝桃素对小鼠生长性能、脏器指数及盲肠微生物多样性的影响[J].畜牧与饲料科学,2022,43(5):7-12. |
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| 作者姓名： | 金丝桃素对小鼠生长性能、脏器指数及盲肠微生物多样性的影响 |
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| 作者单位： | 吉林农业科技学院动物科技学院,吉林 左家 132109 |
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| 基金项目： | 吉林农业科技学院省级大学生科技创新创业训练计划项目（吉农院合字[2022]第SJ2022014号） |
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| 摘 要： | 目的] 探讨金丝桃素对小鼠生长性能、脏器指数及盲肠微生物多样性的影响。方法] 将100只4周龄雌性昆明小鼠随机分为5组,每组20只;金丝桃素通过饮水给予小鼠,添加量分别为0、1、2、4、8 g/L;正饲期28 d,记录小鼠体重和采食情况,计算平均日增重、平均日采食量和料重比;记录死亡小鼠数量,计算死亡率;试验结束时用颈椎脱臼法处死小鼠,采集并称量心脏、肝脏、肾脏和脾脏,称重并计算脏器指数;采集盲肠内容物,利用16S rDNA高通量测序法分析盲肠微生物多样性。结果] 饮水中添加1、2、4 g/L的金丝桃素可提高平均日增重（P>0.05）,4 g/L组平均日增重最高,比0 g/L组增加21.21%;饮水中添加金丝桃素可提高平均日采食量（P>0.05）,2 g/L组平均日采食量最高,比0 g/L组增加18.30%;4 g/L组的料重比最低（P>0.05）,比0 g/L组降低9.24%。1 g/L组、2 g/L组、4 g/L组、8 g/L组的心脏指数显著（P<0.05）高于0 g/L组,4 g/L组的肾脏指数显著（P<0.05）低于1 g/L组。盲肠菌群在门（Phylum）水平上,以厚壁菌门（Firmicutes）相对丰度最高,其次为拟杆菌门（Bacteroidetes）、变形菌门（Proteobacteria）,3个菌门占细菌总数的97.58%。在属（Genus）水平上, 普雷沃菌属（Prevotella）、拟杆菌属（Bacteroides）、考拉杆菌属（Phascolarctobacterium）相对丰度较高;1 g/L组、2 g/L组、4 g/L组的普雷沃菌属（Prevotella）和巨单胞菌属（Megamonas）的相对丰度显著（P<0.05）低于0 g/L组,副瘤胃球菌属（Ruminococcus]）显著（P<0.05)高于0 g/L组;4 g/L组盲肠副拟杆菌属（Parabacteroides）、瘤胃球菌属（Ruminococcus）、乳杆菌属（Lactobacillus）显著（P<0.05)高于0 g/L组。结论] 在饮水中添加金丝桃素在一定程度上有助于提高小鼠采食量和增重,显著提高心脏指数,增加盲肠内有益菌的相对丰度,对小鼠健康表现出有益作用。
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| 关 键 词： | 金丝桃素 小鼠 生长性能 脏器指数 盲肠微生物 |
| 收稿时间： | 2022-06-10 |
Effects of Hypericin on Growth Performance,Organ Index and Cecal Microbial Diversity in Mice |
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| SONG Yu-tong,ZHANG Zi-hao,LYU Hai-xuan,ZHAO Yu-yang,GAO Yi-fan,XU Si-qi,HE Yu-hua,DENG Jia-mei.Effects of Hypericin on Growth Performance,Organ Index and Cecal Microbial Diversity in Mice[J].Animal Husbandry and Feed Science,2022,43(5):7-12. |
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| Authors: | SONG Yu-tong ZHANG Zi-hao LYU Hai-xuan ZHAO Yu-yang GAO Yi-fan XU Si-qi HE Yu-hua DENG Jia-mei |
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| Institution: | College of Animal Science and Technology,Jilin Agricultural Science and Technology University,Zuojia 132109,China |
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| Abstract: | Objective] This study aimed to evaluate the effects of hypericin on growth performance, organ index and cecal microbial diversity in mice. Method] A total of 100 female Kunming mice aged 4 weeks were randomly divided into 5 groups with 20 mice in each group. The mice were given hypericin in drinking water at dosages of 0, 1, 2, 4 and 8 g/L. The formal experiment lasted for 28 days. The body weight and feed intake of the mice were recorded, and the average daily gain, average daily feed intake and feed to gain ratio were calculated. The number of dead mice during the experiment was recorded to calculate the mortality. At the end of the experiment, the mice were killed by cervical dislocation, the heart, liver, kidney and spleen were collected and weighed, and the corresponding organ indexes were determined. The cecal contents were collected and 16S rDNA high-throughput sequencing was used to assess the cecal microbial diversity. Result] Addition of hypericin in drinking water at dosages of 1, 2, 4 g/L increased the average daily gain of mice （P>0.05）, and the highest average daily gain was observed in 4 g/L group, which was elevated by 21.21% compared with that in 0 g/L group. The mice given hypericin in drinking water had higher average daily feed intake （P>0.05）, and the highest average daily feed intake was observed in 2 g/L group, which was elevated by 18.30% compared with that in 0 g/L group. The 4 g/L group had the lowest feed to gain ratio （P>0.05）, which was decreased by 9.24% compared with 0 g/L group. The cardiac index of 1, 2, 4 and 8 g/L groups was significantly （P<0.05） higher than that of 0 g/L group, and the renal index of 4 g/L group was significantly （P<0.05） lower than that of 1 g/L group. At the Phylum level, Firmicutes had the highest relative abundance, followed by Bacteroidetes and Proteobacteria, and the three dominant phyla accounted for 97.58% of the total bacteria. At the Genus level, Prevotella, Bacteroides and Phascolarctobacterium exhibited higher relative abundance; 1, 2 and 4 g/L groups had significantly （P<0.05） lower relative abundance of Prevotella and Megamonas compared with 0 g/L group, and had significantly （P<0.05） higher relative abundance ofRuminococcus]compared with 0 g/L group; 4 g/L group had significantly （P<0.05） higher relative abundance of Parabacteroides, Ruminococcus, and Lactobacillus compared with 0 g/L group. Conclusion] Adding hypericin in drinking water increased the feed intake and weight gain of mice to a certain extent, significantly improved the cardiac index, enhanced the relative abundance of beneficial bacteria in the cecum, showing a beneficial effect on the health of mice. |
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| Keywords: | hypericin mice growth performance organ index cecal microorganism |
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