| 基于线粒体Cytb基因和D-loop区的野生与养殖小黄鱼群体遗传多样性 |
| |
| 引用本文： | 郭丹丹,刘峰,牛宝龙,楼宝.基于线粒体Cytb基因和D-loop区的野生与养殖小黄鱼群体遗传多样性[J].浙江农业学报,2022,34(9):1856. |
| |
| 作者姓名： | 郭丹丹 刘峰 牛宝龙 楼宝 |
| |
| 作者单位： | 浙江省农业科学院 水生生物研究所,浙江 杭州 310021 |
| |
| 基金项目： | 国家重点研发计划“蓝色粮仓科技创新”重点专项(2018YFD0901204);浙江省重点研发计划(2020C02015);浙江省重点研发计划(2021C02055) |
| |
| 摘 要： | 为研究野生与养殖小黄鱼群体的遗传多样性,基于mtDNA Cytb基因和D-loop控制区对舟山嵊泗海域(SS)和象山三门口海域(SMK)2个小黄鱼野生群体和1个养殖群体(YZ)的遗传结构与遗传分化等进行比较分析。序列分析结果显示,Cytb基因序列为841 bp,其A+T含量(50.2%)与C+G含量(49.8%)相似;D-loop区序列为629~635 bp,A+T含量(58.9%)远高于C+G含量(41.1%)。SS、SMK和YZ群体Cytb基因的单倍型数分别为26、27和12,SS和SMK群体共享2个单倍型(Hap1和Hap13),SMK和YZ群体共享1个单倍型(Hap41);SS、SMK和YZ群体D-loop区的单倍型数分别为27、30和10,SS和SMK群体共享1个单倍型(Hap4)。多样性分析结果显示,3个群体均属于高单倍型多样性(Hd>0.5),其中,SS和SMK群体单倍型多样性和核苷酸多样性高于YZ群体,表明野生群体多样性略高于养殖群体。遗传分化指数显示,2个小黄鱼野生群体间的分化程度极小,而养殖群体与野生群体间存在中度分化。遗传分化指数和AMOVA分析结果表明,群体内个体的变异是遗传变异的主要来源。Cytb基因和D-loop区序列中性检验结果中SS和SMK群体的Tajima’s D值和Fu and Li's值均为负数,且Cytb基因的Tajima’s D值和Fu and Li's值显著(P<0.05)偏离中性,表明2个野生群体有可能经历过群体扩张。单倍型系统发育树显示,SS、SMK和YZ群体均未表现出明显的地理聚集,群体间互有交叉,表明3个群体间的分化尚不明显。
|
| 关 键 词： | 小黄鱼 线粒体DNA Cytb基因 D-loop区 遗传多样性 |
| 收稿时间： | 2022-01-04 |
Genetic diversity of wild and cultured populations of little yellow croaker (Larimichthys polyactis) based on mitochondrial Cytb gene and D-loop region |
| |
| GUO Dandan,LIU Feng,NIU Baolong,LOU Bao.Genetic diversity of wild and cultured populations of little yellow croaker (Larimichthys polyactis) based on mitochondrial Cytb gene and D-loop region[J].Acta Agriculturae Zhejiangensis,2022,34(9):1856. |
| |
| Authors: | GUO Dandan LIU Feng NIU Baolong LOU Bao |
| |
| Institution: | Institute of Hydrobiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
| |
| Abstract: | In order to study the genetic diversity of wild and cultured populations of little yellow croaker (Larimichthys polyactis), the genetic structure and differentiation of two wild (SS and SMK) and one cultured (YZ) populations of little yellow croaker was analyzed based on mitochondrial DNA Cytb gene and D-loop control region. The results showed that the sequences of Cytb gene were 841 bp, with A+T (50.2%) content similar to C+G (49.8%) content. The D-loop sequences were 629-635 bp, and A+T content (58.9%) was significantly higher than C+G content (41.1%). Based on the analysis of Cytb gene, the haplotypes of SS, SMK and YZ populations were 26, 27 and 12, respectively. SS and SMK populations shared two haplotypes (hap1 and hap13), SMK and YZ populations shared one haplotypes (hap41). Based on D-loop, the haplotypes of SS, SMK and YZ populations were 27, 30 and 10, respectively, and SS and SMK populations shared one haplotype (Hap4). The diversity results showed that the haplotype diversity (Hd>0.5) of the three populations were high. In addition, the haplotype diversity and nucleotide diversity of SS and SMK populations were slightly higher than those of YZ population, indicating that the diversity of wild populations was higher than that of cultured population. The results of fixation index and AMOVA showed that the genetic variation was mainly derived from individual variation within the population, the genetic differentiation between populations of SS and SMK was small, but there was a moderate differentiation between the cultured and wild population. Neutral test analysis of SS and SMK populations showed that Tajima’s D and Fu and Li's value were both negative, and significantly (P<0.05) deviated from neutral based on Cytb gene, indicating that both wide populations have undergone a recent population expansion. The haplotype phylogenetic tree was shown that SS, SMK and YZ populations did not formed monophyletic group, which indicated that the genetic differentiation of the three populations was not significant. |
| |
| Keywords: | Larimichthys polyactis mitochondrial DNA Cytb gene D-loop region genetic diversity |
|
| 点击此处可从《浙江农业学报》浏览原始摘要信息 |
| 点击此处可从《浙江农业学报》下载免费的PDF全文 |
|
